Document Detail

The essential fatty acid requirement for azoxymethane-induced intestinal carcinogenesis in rats.
MedLine Citation:
PMID:  2755311     Owner:  NLM     Status:  MEDLINE    
The essential fatty acid requirement for the development of intestinal carcinogenesis was determined and compared to the overall essential fatty acid status of the animals as measured by the triene/tetraene ratio in the plasma, liver and colon. To induce tumors, male Sprague-Dawley rats were given two weekly injections (20 mg/kg body wt) of azoxymethane. Two weeks after the last injection, the rats were divided into groups of 25 and given one of six diets containing various levels of essential fatty acids (as linoleate). The diets contained 5% total fat and were prepared by mixing safflower oil (high essential fatty acids, beef fat (low essential fatty acids), and medium chain triglyceride oil (no essential fatty acids). One group of rats was fed a 20% beef fat diet. The range of essential fatty acids was from less than 0.03% to 1.28% (w/w). Twenty-six weeks after the first azoxymethane injection, the animals were killed and intestinal tumor incidence and multiplicity were determined. Samples of plasma, liver and colon were also taken for measurement of the triene/tetraene ratio by gas chromatography. Large bowel tumor incidence showed a dependence on the essential fatty acid content of the diet. The results were as follows: (percent essential fatty acids: percent tumor incidence) Group A (1.28: 72.4), Group B (0.60: 73.3), Group C (0.11: 55.2), Group D (0.08: 39.3), Group E (less than 0.03: 37.9) and Group F, which was fed 20% beef fat, (0.34: 88.5).(ABSTRACT TRUNCATED AT 250 WORDS)
A W Bull; J C Bronstein; N D Nigro
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  24     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-08-29     Completed Date:  1989-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  340-6     Citation Subset:  IM    
Department of Internal Medicine, Wayne State University, School of Medicine, Detroit, MI 48226.
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MeSH Terms
Arachidonic Acids / metabolism
Azo Compounds / toxicity*
Azoxymethane / toxicity*
Body Weight / drug effects
Dietary Fats, Unsaturated / administration & dosage,  adverse effects
Fatty Acids, Essential / administration & dosage,  adverse effects*
Intestinal Neoplasms / chemically induced*,  epidemiology
Lipids / analysis
Rats, Inbred Strains
Reg. No./Substance:
0/Arachidonic Acids; 0/Azo Compounds; 0/Dietary Fats, Unsaturated; 0/Fatty Acids, Essential; 0/Lipids; 25843-45-2/Azoxymethane

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