Document Detail


An enzymic method of preparing plant chromosomes for in situ hybridization.
MedLine Citation:
PMID:  3952778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method of preparing chromosomes from plant root tips for in situ hybridization with tritiated DNA is described. The technique relies on the enzymic hydrolysis of plant cell walls with a pectinase-cellulase mixture. It is shown that, despite the enzymic mixture possessing nuclease activity, there is no detectable degradation of DNA within fixed root tips. To demonstrate the suitability of this method of preparing plant chromosomes for in situ hybridization, a cloned repetitive DNA sequence has been hybridized to Allium sativum chromosomes. Chromosomes prepared using this technique also can be readily C-banded.
Authors:
G Jamieson; I J Evans; S R Barnes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stain technology     Volume:  61     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-04-24     Completed Date:  1986-04-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  21-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosomes / ultrastructure*
DNA / analysis,  genetics
Nucleic Acid Hybridization
Plants / cytology,  genetics*
Tritium
Chemical
Reg. No./Substance:
10028-17-8/Tritium; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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