Document Detail


The enzymes of sialic acid biosynthesis.
MedLine Citation:
PMID:  15888312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sialic acids are a family of nine carbon alpha-keto acids that play a wide variety of biological roles in nature. In mammals, they are found at the distal ends of cell surface glycoconjugates, and thus are major determinants of cellular recognition and adhesion events. In certain strains of pathogenic bacteria, they are found in capsular polysaccharides that mask the organism from the immune system by mimicking the exterior of a mammalian cell. This review outlines recent developments in the understanding of the two main enzymes responsible for the biosynthesis of the sialic acid, N-acetylneuraminic acid. The first, a hydrolyzing UDP-N-acetylglucosamine 2-epimerase, generates N-acetylmannosamine and UDP from UDP-N-acetylglucosamine. The second, sialic acid synthase, generates either N-acetylneuraminic acid (bacteria) or N-acetylneuraminic acid 9-phosphate (mammals) in a condensation reaction with phosphoenolpyruvate. An emphasis is placed on an understanding of the mechanistic and structural features of these enzymes.
Authors:
Martin E Tanner
Publication Detail:
Type:  Journal Article; Review     Date:  2005-02-25
Journal Detail:
Title:  Bioorganic chemistry     Volume:  33     ISSN:  0045-2068     ISO Abbreviation:  Bioorg. Chem.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-12     Completed Date:  2005-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1303703     Medline TA:  Bioorg Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  216-28     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of British Columbia, Vancouver, BC, Canada V6T 1Z1. mtanner@chem.ubc.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrate Conformation
Carbohydrate Epimerases / chemistry,  metabolism
Hydrolysis
Oxo-Acid-Lyases / chemistry,  metabolism*
Sialic Acids / biosynthesis*
Chemical
Reg. No./Substance:
0/Sialic Acids; EC 2.5.1.56/N-acetylneuraminate synthase; EC 4.1.3.-/Oxo-Acid-Lyases; EC 5.1.3.-/Carbohydrate Epimerases; EC 5.1.3.14/UDP acetylglucosamine-2-epimerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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