Document Detail


An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis.
MedLine Citation:
PMID:  19833868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The polyunsaturated fatty acids (PUFAs) linoleic acid (18:2) and alpha-linolenic acid (18:3) in triacylglycerols (TAG) are major factors affecting the quality of plant oils for human health, as well as for biofuels and other renewable applications. These PUFAs are essential fatty acids for animals and plants, but also are the source of unhealthy trans fats during the processing of many foodstuffs. PUFAs 18:2 and 18:3 are synthesized in developing seeds by the desaturation of oleic acid (18:1) esterified on the membrane lipid phosphatidylcholine (PC) on the endoplasmic reticulum. The reactions and fluxes involved in this metabolism are incompletely understood, however. Here we show that a previously unrecognized enzyme, phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT), encoded by the Arabidopsis ROD1 gene, is a major reaction for the transfer of 18:1 into PC for desaturation and also for the reverse transfer of 18:2 and 18:3 into the TAG synthesis pathway. The PDCT enzyme catalyzes transfer of the phosphocholine headgroup from PC to diacylglycerol, and mutation of rod1 reduces 18:2 and 18:3 accumulation in seed TAG by 40%. Our discovery of PDCT is important for understanding glycerolipid metabolism in plants and other organisms, and provides tools to modify the fatty acid compositions of plant oils for improved nutrition, biofuel, and other purposes.
Authors:
Chaofu Lu; Zhanguo Xin; Zhonghai Ren; Martine Miquel; John Browse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-10-15
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  106     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-05     Completed Date:  2009-12-07     Revised Date:  2010-09-28    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18837-42     Citation Subset:  IM    
Affiliation:
Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Arabidopsis / enzymology*,  genetics*
Arabidopsis Proteins / chemistry,  genetics*
Crosses, Genetic
Fatty Acids / analysis
Genes, Plant*
Isotope Labeling
Molecular Sequence Data
Mutation / genetics
Phosphatidylcholines / metabolism
Phosphotransferases / genetics*,  metabolism
Phylogeny
Seeds / chemistry
Sequence Analysis, Protein
Transferases (Other Substituted Phosphate Groups) / chemistry,  genetics*
Triglycerides / biosynthesis,  metabolism*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Fatty Acids; 0/Phosphatidylcholines; 0/Triglycerides; EC 2.7.-/Phosphotransferases; EC 2.7.8.-/ROD1 protein, Arabidopsis; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups)
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