Document Detail


An enzyme-linked immunosorbent assay for quantitation of Haemophilus Influenzae type b polysaccharide-specific IgG1 and IgG2 in human and infant rhesus monkey sera.
MedLine Citation:
PMID:  1806588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An enzyme-linked immunosorbent assay (ELISA) has been developed and validated to quantitate IgG1 and IgG2 antibody to polyribosyl-ribitol phosphate (PRP), the capsular polysaccharide of Haemophilus influenzae type b (Hib). The sera of children and infant Rhesus monkeys immunized with an Hib conjugate vaccine composed of Hib PRP covalently linked to an outer membrane protein complex (OMPC) from Neisseria meningitidis serogroup B (PedvaxHIB, PRP-OMPC, Merck, Sharp and Dohme Research Laboratories). The solid-phase antigen employed in the ELISA is a conjugate of PRP to human serum albumin. The enzyme-labeled antibody is alkaline phosphatase-conjugated mouse monoclonal (mAb) anti-human IgG1 or IgG2. A human serum standard was calibrated using parallel titrations with a known antibody standard. The geometric mean titer (GMT) of the anti-PRP IgG1 response to one dose of PedvaxHIB was 3.87 micrograms/ml (n = 82), 11.80 micrograms/ml (n = 62) and 14.57 micrograms/ml (n = 74) in infants and children 12 to 17 months, 18 to 23 months and greater than or equal to 24 months old, respectively. Infants 2 to 11 months old responded with an IgG1 anti-PRP response of 7.10 micrograms/ml while infant monkeys responded with a GMT of 150.65 (n = 9) after two doses of vaccine. The anti-PRP IgG2 GMT responses in all groups were less than 0.25 micrograms/ml, except for humans greater than or equal to 18-months old who exhibited a GMT of greater than or equal to 0.40 micrograms/ml (n = 75). PedvaxHIB, immunization of human infants and children and infant Rhesus monkeys elicits primarily an IgG1 response to PRP. The monkey model appears to be a reliable indicator of the human immune response.
Authors:
K T Dolan; J M Staub; T L Schofield; V I Ahonkhai; R W Ellis; P P Vella
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Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Journal of immunoassay     Volume:  12     ISSN:  0197-1522     ISO Abbreviation:  J Immunoassay     Publication Date:  1991  
Date Detail:
Created Date:  1992-05-08     Completed Date:  1992-05-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8007167     Medline TA:  J Immunoassay     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  543-64     Citation Subset:  IM    
Affiliation:
Department of Cellular and Molecular Biology, Merck Sharp and Dohme Research Laboratories, West Point, PA 19486.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Anti-Idiotypic / immunology
Antibodies, Bacterial / blood*,  immunology
Antibodies, Monoclonal / immunology
Bacterial Outer Membrane Proteins / immunology*
Bacterial Vaccines / immunology*
Child, Preschool
Enzyme-Linked Immunosorbent Assay*
Haemophilus Vaccines*
Haemophilus influenzae / immunology
Humans
Immunization
Immunoglobulin G / blood*
Infant
Macaca mulatta / blood*,  immunology
Neisseria meningitidis / immunology
Polysaccharides, Bacterial / immunology*
Sensitivity and Specificity
Vaccines, Synthetic / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Antibodies, Bacterial; 0/Antibodies, Monoclonal; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Vaccines; 0/Haemophilus Vaccines; 0/Haemophilus influenzae-type b polysaccharide-Neisseria meningitidis outer membrane protein conjugate vaccine; 0/Immunoglobulin G; 0/Polysaccharides, Bacterial; 0/Vaccines, Synthetic

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