Document Detail


An enzyme-linked immunosorbent assay for the detection of antitetanus toxoid antibody using aluminum-absorbed coating antigen.
MedLine Citation:
PMID:  2313470     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed tetanus suspension. The specificity of the antibodies for tetanus antigen was tested both before and after antitetanus activity was removed with tetanus antigen-coated beads. Also, the activities of the sera were compared with those of a commercially available test. Our results indicated that the aluminum-absorbed tetanus suspension can be coated onto standard polystyrene ELISA plates and used to measure antibody titers to tetanus toxoid.
Authors:
G M Thiele; J Rogers; M Collins; N Yasuda; D Smith; T L McDonald
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical laboratory analysis     Volume:  4     ISSN:  0887-8013     ISO Abbreviation:  J. Clin. Lab. Anal.     Publication Date:  1990  
Date Detail:
Created Date:  1990-04-26     Completed Date:  1990-04-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8801384     Medline TA:  J Clin Lab Anal     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  126-9     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University of Nebraska Medical Center, Omaha 68105-1065.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Aluminum
Antibodies, Bacterial / blood*
Antigens, Bacterial
Enzyme-Linked Immunosorbent Assay*
Evaluation Studies as Topic
Humans
Immunoglobulin M / biosynthesis
Tetanus Toxoid / immunology*
Grant Support
ID/Acronym/Agency:
CA30196/CA/NCI NIH HHS; CA36727/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Immunoglobulin M; 0/Tetanus Toxoid; 7429-90-5/Aluminum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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