Document Detail


An enzyme-P.M.R.-spectroscopic determination of the enantiomers of galactose.
MedLine Citation:
PMID:  902277     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The action of D-galactose oxidase on D-galactose in the presence of oxygen afforded meso-galacto-hexodialdose quantitatively, which allowed p.m.r.-spectroscopic determination of the D enantiomer in a DL mixture. In the spectrum of the products obtained from the enzymic treatment of a mixture of D- and L-galactose, the magnitude of the aldehydrol signals derived solely from the oxidised D enantiomer, relative to those of the anomeric signals, provided the fractional content of the D enantiomer. This simple, accurate, and convenient procedure was applied to the hydrolysate of a seaweed galactan, which was also analyzed, for comparative purposes, by the fermentation technique employing D-galactose-adapted Saccharomyces cerevisiae.
Authors:
J N Whyte; J R Englar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  57     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-11-25     Completed Date:  1977-11-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  273-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Galactose*
Galactose Oxidase*
Isomerism
Kinetics
Magnetic Resonance Spectroscopy
Molecular Conformation
Substrate Specificity
Chemical
Reg. No./Substance:
26566-61-0/Galactose; EC 1.1.3.9/Galactose Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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