| The enzymatic phosphorylation of nucleic acids and its application to end-group analysis. | |
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MedLine Citation:
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PMID: 5338564 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polynucleotide kinase catalyzes the transfer of a phosphate group from ATP to the 5'-hydroxyl termini of polynucleotides. Selective labeling of the 5'-hydroxyl termini of DNA with polynucleotide kinase has been used to study the number and the identity of the 5'-terminal residues of bacteriophage DNA's, and to examine the nature of the phosphodiester bond cleavages produced by endonucleases and by sonic irradiation. The intact strands of T7 DNA bear 5'-phosphoryl end-groups; only deoxyadenylate and deoxythymidylate are present as 5'-terminal residues. The intact strands of native lambda-DNA bear 5'-hydroxyl end-groups. M13 DNA, a circular molecule, cannot be phosphorylated. End-group labeling of DNA provides a method for determination of molecular weight; calibration against other DNA preparations is not required. The molecular weight of a single strand of T7 DNA, determined by end-group labeling, is 13.1 x 10(6); the molecular weight of a single strand of lambda-DNA is 16.0 x 10(6). These values are in agreement with molecular weight estimates by sedimentation analysis and electron microscopy. Sonic irradiation of DNA has been shown to favor the production of polynucleotides terminated by 5'-phosphomonoester groups. All four deoxyribonucleotides are present as 5'-terminal residues of sonicated DNA. |
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Authors:
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C C Richardson; B Weiss |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of general physiology Volume: 49 ISSN: 0022-1295 ISO Abbreviation: J. Gen. Physiol. Publication Date: 1966 Jul |
Date Detail:
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Created Date: 1967-08-14 Completed Date: 1967-08-14 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 2985110R Medline TA: J Gen Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 81-97 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate Alkaline Phosphatase Bacteriophages Chemical Phenomena Chemistry Coliphages DNA, Viral* Deoxyribonucleases Escherichia coli / enzymology Molecular Weight Phosphotransferases Polynucleotides* Ultrasonics |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Polynucleotides; 56-65-5/Adenosine Triphosphate; EC 2.7.-/Phosphotransferases; EC 3.1.-/Deoxyribonucleases; EC 3.1.3.1/Alkaline Phosphatase |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Gen Physiol ISSN: 0022-1295 ISSN: 1540-7748 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1966 by The Rockefeller University Press Print publication date: Day: 1 Month: 7 Year: 1966 Volume: 49 Issue: 6 First Page: 81 Last Page: 97 ID: 2195536 PubMed Id: 5338564 |
| The Enzymatic Phosphorylation of Nucleic Acids and Its Application to End-Group Analysis | |
| Charles C. Richardson | |
| Bernard Weiss | |
| From the Department of Biological Chemistry, Harvard Medical School, Boston |
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