| An enzymatic method for calibration of serum lipase assays. | |
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MedLine Citation:
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PMID: 3948404 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An enzymatic method for the calibration of a turbidimetric lipase assay is described, based on measurement of free fatty acids liberated by the action of lipase. The substrate of the turbidimetric assay is a colipase-containing triolein emulsion. For determination of the free fatty acids a commercial test kit including acyl-CoA synthetase, acyl-CoA oxidase, and peroxidase is used. Intra- and interassay imprecision (CV) is about 5% at above-normal lipase activities, about 10% at normal values. Temperature coefficients are 1.24 and 1.45, respectively, for measurements at 30 and 37 degrees C vs 25 degrees C. |
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Authors:
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G E Hoffmann; U Neumann; S Hoffmann; P Kaspar; L Weiss |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical chemistry Volume: 32 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1986 Mar |
Date Detail:
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Created Date: 1986-04-03 Completed Date: 1986-04-03 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 545-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calibration Fatty Acids, Nonesterified / analysis* Humans Lipase / blood* Nephelometry and Turbidimetry Swine Temperature |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Nonesterified; EC 3.1.1.3/Lipase |
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