| An enzymatic assay for the colorimetric and fluorimetric determination of uric acid in sera. | |
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MedLine Citation:
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PMID: 2360868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The methods described in this paper are based on the uricase catalyzed oxidation of uric acid to allantoine and hydrogen peroxide. By making use of the catalytic activity of peroxidase the generated H2O2 is measured either spectrophotometrically with 3-methyl-benzothiazoline-2-one hydrazone (MBTH) and 3-dimethylaminobenzoic acid (DMAB) (M1) or fluorimetrically with tyramine (M2) or L-tyrosine (M3). The methods are simple, sensitive and selective. The procedures developed can be rapidly and readily performed on patient serum samples without deproteinization using 100 microliters and 5 microliters for colorimetric and fluorimetric assay, respectively. |
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Authors:
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K A Kovar; M N el Bolkiny; R Rink; M A Hamid |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archiv der Pharmazie Volume: 323 ISSN: 0365-6233 ISO Abbreviation: Arch. Pharm. (Weinheim) Publication Date: 1990 Apr |
Date Detail:
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Created Date: 1990-08-02 Completed Date: 1990-08-02 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0330167 Medline TA: Arch Pharm (Weinheim) Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 235-7 Citation Subset: IM |
Affiliation:
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Pharmazeutisches Institut der Universität Tübingen, FRG. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Colorimetry Humans Spectrometry, Fluorescence Uric Acid / blood* |
| Chemical | |
Reg. No./Substance:
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69-93-2/Uric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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