Document Detail


An enzymatic assay for the colorimetric and fluorimetric determination of uric acid in sera.
MedLine Citation:
PMID:  2360868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The methods described in this paper are based on the uricase catalyzed oxidation of uric acid to allantoine and hydrogen peroxide. By making use of the catalytic activity of peroxidase the generated H2O2 is measured either spectrophotometrically with 3-methyl-benzothiazoline-2-one hydrazone (MBTH) and 3-dimethylaminobenzoic acid (DMAB) (M1) or fluorimetrically with tyramine (M2) or L-tyrosine (M3). The methods are simple, sensitive and selective. The procedures developed can be rapidly and readily performed on patient serum samples without deproteinization using 100 microliters and 5 microliters for colorimetric and fluorimetric assay, respectively.
Authors:
K A Kovar; M N el Bolkiny; R Rink; M A Hamid
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archiv der Pharmazie     Volume:  323     ISSN:  0365-6233     ISO Abbreviation:  Arch. Pharm. (Weinheim)     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-08-02     Completed Date:  1990-08-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0330167     Medline TA:  Arch Pharm (Weinheim)     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  235-7     Citation Subset:  IM    
Affiliation:
Pharmazeutisches Institut der Universität Tübingen, FRG.
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MeSH Terms
Descriptor/Qualifier:
Colorimetry
Humans
Spectrometry, Fluorescence
Uric Acid / blood*
Chemical
Reg. No./Substance:
69-93-2/Uric Acid

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