Document Detail

An environmentally relevant mixture of organochlorines, their metabolites and effects on preimplantation development of porcine embryos.
MedLine Citation:
PMID:  18479888     Owner:  NLM     Status:  MEDLINE    
Environmental exposure of human populations to organochlorines is still widespread despite several international regulations banning or restricting their use. This study tested the hypothesis that an environmentally relevant complex mixture of organochlorines comprising polychlorinated biphenyls (PCBs), technical chlordane, dichlorodiphenyldichloroethylene and 12 other components is toxic for porcine embryos (at relative concentrations of 1-10000-fold the environmental organochlorine levels of contamination or 4.2 microg/l total PCBs). We also tested the embryotoxicity of a metabolised organochlorine mixture (relative concentrations of 0.9, 1.8, 2.7, 3.6 and 4.5 microg/l hydroxy-PCBs (OH-PCBs)) obtained by extracting plasma samples from sows treated with the native mixture. Embryos produced in vitro were exposed to either the organochlorine mixture or the metabolised extract for 9 days. The organochlorine mixture reduced embryonic development at the 10000x concentration (relative concentration of 42 mg/l PCBs; p=0.05). The organochlorine mixture also reduced the mean number of blastomeres per expanded blastocyst in a dose-dependent manner (p=0.038) but did not induce blastomere apoptosis (p>0.05). In contrast, the metabolised extract did not affect development or blastomere number at the concentrations tested, although the highest level of this mixture (4.5 microg/l OH-PCBs) was still very low (i.e. similar to the 1x concentration of the organochlorine mixture, which also did not alter embryo parameters). These data lead to the conclusion that while high concentrations of the native organochlorine mixture are toxic for porcine embryos, concentrations of either the native or the metabolised mixture that bear some relevance to exposure of human populations in the Arctic were without observable effect.
Céline Campagna; Pierre Ayotte; Marc-André Sirard; Janice L Bailey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-27
Journal Detail:
Title:  Reproductive toxicology (Elmsford, N.Y.)     Volume:  25     ISSN:  0890-6238     ISO Abbreviation:  Reprod. Toxicol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-26     Completed Date:  2008-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803591     Medline TA:  Reprod Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  361-6     Citation Subset:  IM    
Centre de recherche en biologie de la reproduction, Département des sciences animales, Pavillon Paul-Comtois, Université Laval, Ste-Foy, Québec, QC, Canada G1V 0A6.
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MeSH Terms
Blastocyst / drug effects*,  physiology
Chlordan / toxicity
Dichlorodiphenyl Dichloroethylene / toxicity
Embryonic Development / drug effects
Environmental Pollutants / toxicity*
Hydrocarbons, Chlorinated / toxicity*
Oocysts / drug effects
Polychlorinated Biphenyls / toxicity
Reg. No./Substance:
0/Environmental Pollutants; 0/Hydrocarbons, Chlorinated; 0/Polychlorinated Biphenyls; 12789-03-6/Chlordan; 72-55-9/Dichlorodiphenyl Dichloroethylene

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