Document Detail

The environment of tryptophan in pig pancreatic phospholipase A2 bound to bilayers.
MedLine Citation:
PMID:  3978097     Owner:  NLM     Status:  MEDLINE    
Binding of pig pancreatic phospholipase A2 to ternary codispersions of diacylphosphatidylcholine/lysophosphatidylcholine/fatty acid (100:22:22, mole ratio) is monitored by the increase in intrinsic fluorescence intensity of the single tryptophan residue. The fluorescence is quenched by the brominated fatty acid components in the ternary codispersions. The quenching efficiency is in the order: 11,12-dibromo- greater than 9,10-dibromo- greater than 6,7-dibromo- greater than 2-bromo fatty acid. The quenching efficiency of the 9,10-brominated derivatives of the three components in the ternary codispersions is in the order diacylphosphatidylcholine greater than fatty acid greater than lysophosphatidylcholine. Two isomers of diacylphosphatidylcholine with 9,10-dibromo substituents on chain 1 or 2 are equally efficient quenchers. While succinimide also quenches the fluorescence of the free and the membrane bound enzyme, the tryptophan residue in both systems is not accessible to 1-methylnicotinamide. These results are rationalized by a hypothesis that the acyl chains of the substrate interacts with the tryptophan residue of pig pancreatic phospholipase A2, which is readily accessible to water soluble neutral quenchers both in the free and the bound state.
M K Jain; B P Maliwal
Related Documents :
3943917 - Tolerance to the trinitrophenol ligand in guinea pigs: studies on the role of the solve...
6412317 - Release of leukotriene c4 from guinea pig trachea.
7469887 - Comparative ototoxicity of chloramphenicol and kanamycin with ethacrynic acid.
11108547 - The uterotonic activity of compounds isolated from the supercritical fluid extract of e...
15977 - Extracellular phosphatases of chlamydomonas reinhardi and their regulation.
6154697 - The effect of amino acid starvation on nucleoside uptake and rna synthesis in tetrahymena.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  814     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-05-20     Completed Date:  1985-05-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  135-40     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Lipid Bilayers / metabolism*
Pancreas / enzymology*
Phospholipases / metabolism*
Phospholipases A / metabolism*
Phospholipases A2
Grant Support
Reg. No./Substance:
0/Lipid Bilayers; 73-22-3/Tryptophan; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Interaction of human erythrocyte Band 3 with Ricinus communis agglutinin and other lectins.
Next Document:  Mechanisms of uptake of ketone bodies by luminal-membrane vesicles.