Document Detail


The enigmatic thymine DNA glycosylase.
MedLine Citation:
PMID:  17116428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When it was first isolated from extracts of HeLa cells in Josef Jiricny's laboratory, the thymine DNA glycosylase (TDG) attracted attention because of its ability to remove thymine, i.e. a normal DNA base, from G.T mispairs. This implicated a function of DNA base excision repair in the restoration of G.C base pairs following the deamination of a 5-methylcytosine. TDG turned out to be the founding member of a newly emerging family of mismatch-directed uracil-DNA glycosylases, the MUG proteins, that act on a comparably broad spectrum of base lesion including G.U as the common, most efficiently processed substrate. However, because of its apparent catalytic inefficiency, some have considered TDG a poor DNA repair enzyme without an important biological function. Others have reported 5-meC DNA glycosylase activity to be associated with TDG, thrusting the enzyme into limelight as a possible DNA demethylase. Yet others have found the glycosylase to interact with transcription factors, implicating a function in gene regulation, which appears to be critically important in developmental processes. This article reviews all these developments in view of possible biological functions of this multifaceted DNA glycosylase.
Authors:
Daniel Cortázar; Christophe Kunz; Yusuke Saito; Roland Steinacher; Primo Schär
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2006-11-20
Journal Detail:
Title:  DNA repair     Volume:  6     ISSN:  1568-7864     ISO Abbreviation:  DNA Repair (Amst.)     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-12     Completed Date:  2007-05-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139138     Medline TA:  DNA Repair (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  489-504     Citation Subset:  IM    
Affiliation:
Centre for Biomedicine, Department of Clinical Biological Research, University of Basel, Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
DNA Mismatch Repair*
DNA Repair*
Evolution, Molecular
Humans
Molecular Sequence Data
Protein Conformation
Sequence Alignment
Thymine DNA Glycosylase / chemistry,  genetics,  metabolism*
Chemical
Reg. No./Substance:
EC 3.2.2.-/Thymine DNA Glycosylase; EC 3.2.2.-/mismatch-specific thymine uracil-DNA glycosylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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