Document Detail

The enhancing role of vitamin A deficiency on chemically induced nephroblastoma in rats.
MedLine Citation:
PMID:  16338327     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Vitamin A plays an important role in the prevention of neoplasia. The occurrence of nephroblastoma, a common renal malignancy of childhood, is said to be closely correlated with retardation of differentiation of metanephric blastema. The aim of this study was to clarify the effect of vitamin A deficiency (VAD) in experimental nephroblastoma. METHODS: Sixty weaning outbred female Wistar rats weighing 40 to 50 g were injected with 0.35% dimethylhydrazine dihydrochloride (DMH) subcutaneously and divided into 2 groups. Animals were fed with diets containing vitamin A, normal diet (ND group; n = 30), and vitamin A-deficient diet (VAD group; n = 30). Serum concentrations of vitamin A on day 120 after DMH injection were assayed. The incidence of nephroblastomas in 2 groups was recorded. The renal tissues were prepared for morphological studies and WT1 messenger RNA (mRNA) detection on day 365 after DMH injection. RESULTS: The average serum concentrations of vitamin A in the ND group (2.60 +/- 0.53 mumol/L) were significantly higher than that in the VAD group (0.51 +/- 0.26 mumol/L, P < .05) 120 days after DMH injection. On day 365, the incidence of renal tumors in the VAD group (36.7%) was significantly higher than that of the ND group (13.3%, P < .05). Certain histological and ultrastructural features and WT1 mRNA expression patterns observed in these tumors showed that they had a resemblance to human nephroblastomas. Nephrogenic rests (NRs) could be seen in the adjacent normal renal parenchyma. The incidence of NRs in the VAD group (16.7%) was significantly higher than that in the ND group (3.3%, P < .05). The mutant WT1 mRNA expression levels in the VAD group (0.75 +/- 0.11) were significantly higher than those in the ND group (0.24 +/- 0.06, P < .05). CONCLUSIONS: Vitamin A deficiency can increase development of nephroblastomas in rats, probably via a mechanism of elevated NR remnants and WT1 expression.
Kai Li; Jiechun Gao; Xianmin Xiao; Lian Chen; Liang Zhang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  40     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-12     Completed Date:  2006-07-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1951-6     Citation Subset:  IM    
Department of Surgery, Children's Hospital, Medical Center of Fudan University (Former Shanghai Medical University), Shanghai 200032, PR China.
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MeSH Terms
Kidney Neoplasms / chemically induced*,  physiopathology*
Rats, Wistar
Vitamin A / blood
Vitamin A Deficiency / complications*
WT1 Proteins / genetics,  physiology
Wilms Tumor / chemically induced*,  physiopathology*
Reg. No./Substance:
0/WT1 Proteins; 11103-57-4/Vitamin A

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