| The enhancement of osteogenesis by nano-fibrous scaffolds incorporating rhBMP-7 nanospheres. | |
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MedLine Citation:
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PMID: 17239946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is advantageous to incorporate controlled growth factor delivery into tissue engineering strategies. The objective of this study was to develop a three-dimensional (3D) porous tissue engineering scaffold with the capability of controlled releasing recombinant human bone morphogenetic protein-7 (rhBMP-7) for enhancement of bone regeneration. RhBMP-7 was first encapsulated into poly(lactic-co-glycolic acid) (PLGA) nanospheres (NS) with an average diameter of 300nm. Poly(l-lactic acid) (PLLA) scaffolds with interconnected macroporous and nano-fibrous architectures were prepared using a combined sugar sphere template leaching and phase separation technique. A post-seeding technique was then utilized to immobilize rhBMP-7 containing PLGA nanospheres onto prefabricated nano-fibrous PLLA scaffolds with well-maintained 3D structures. In vitro release kinetics indicated that nanosphere immobilized scaffold (NS-scaffold) could release rhBMP-7 in a temporally controlled manner, depending on the chemical and degradation properties of the NS which were immobilized onto the scaffold. In vivo, rhBMP-7 delivered from NS-scaffolds induced significant ectopic bone formation throughout the scaffold while passive adsorption of rhBMP-7 into the scaffold resulted in failure of bone induction due to either the loss of rhBMP-7 biological function or insufficient duration within the scaffold. We conclude that the interconnected macroporous architecture and the sustained, prolonged delivery of bioactive rhBMP-7 from NS immobilized nano-fibrous scaffolds actively induced new bone formation throughout the scaffold. The approach offers a new delivery method of BMPs and a novel scaffold design for bone regeneration. |
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Authors:
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Guobao Wei; Qiming Jin; William V Giannobile; Peter X Ma |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-01-08 |
Journal Detail:
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Title: Biomaterials Volume: 28 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-02-09 Completed Date: 2007-05-22 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 2087-96 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109-2209, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins / metabolism* Humans Kinetics Microscopy, Electron, Scanning Nanotubes* Osteogenesis* Recombinant Proteins / metabolism Tissue Engineering Transforming Growth Factor beta / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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DE13397/DE/NIDCR NIH HHS; DE14755/DE/NIDCR NIH HHS; DE15384/DE/NIDCR NIH HHS; R01 DE013397-04/DE/NIDCR NIH HHS; R01 DE013397-05/DE/NIDCR NIH HHS; R01 DE015384-01/DE/NIDCR NIH HHS; R01 DE015384-02/DE/NIDCR NIH HHS; R01 DE015384-03/DE/NIDCR NIH HHS; R01 DE015384-04/DE/NIDCR NIH HHS; R21 DE014755-01A1/DE/NIDCR NIH HHS; R21 DE014755-02/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/BMP7 protein, human; 0/Bone Morphogenetic Protein 7; 0/Bone Morphogenetic Proteins; 0/Recombinant Proteins; 0/Transforming Growth Factor beta |
| Comments/Corrections | |
Comment In:
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Nanomedicine (Lond). 2007 Jun;2(3):385-90
[PMID:
17716183
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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