| The enhancement of immunosuppressive effects of cyclosporine A on human T-cells using fusogenic liposomes. | |
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MedLine Citation:
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PMID: 18930640 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to prepare and characterize neutral, positively charged, negatively charged and fusogenic liposomes of different sizes that contain cyclosporine A (CyA) and to evaluate their immunosuppressive activity on human T-cells. Neutral liposomes containing CyA were prepared from dipalmitoylphosphatidylcholine (DPPC) and cholesterol using the solvent evaporation method. To prepare positively charged, negatively charged and fusogenic liposomes containing CyA; stearylamine (SA), dicetylphosphate (DCP) and dioleoylphosphatidylethanolamine (DOPE) were added to the neutral liposome formulation, respectively. To reduce the size of liposomes containing CyA, extrusion through polycarbonate filters (1000, 400 and 100 nm) was used. The liposomes were characterized by their size, zeta potential and encapsulation efficiency. The in vitro immunosuppressive effects of an aqueous solution of CyA and different liposomes containing CyA were determined on human T-cells by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The mean diameter of the various multilamellar vesicle (MLV) liposomes containing CyA was between 1.76 and 2.49 microm. The encapsulation efficiency for the different MLV and extruded liposomes containing CyA ranged from 73% to 90%. In vitro immunosuppressive evaluation by T-cell culture showed that fusogenic liposomes have the best inhibitory effects on T-cell proliferation compared to the other liposomes. Reducing the size of the liposomes did not affect the in vitro immunosuppressive activity. The average IC(50) for the aqueous solution of CyA and the neutral, positively charged, negatively charged and fusogenic liposomes containing CyA was 4.98 x 10(-2), 7.38, 1.43, 3.84 x 10(-3) and 7.93 x 10(-5) mM, respectively. The results of this study indicate that fusogenic liposomes have the strongest immunosuppressive activity and could be considered as a suitable delivery system for CyA. |
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Authors:
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Bizhan Malaekeh-Nikouei; Mahmoud R Jaafari; Sayyed A Sajadi Tabassi; Afshin Samiei |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-10 |
Journal Detail:
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Title: Colloids and surfaces. B, Biointerfaces Volume: 67 ISSN: 1873-4367 ISO Abbreviation: Colloids Surf B Biointerfaces Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-10 Completed Date: 2009-04-20 Revised Date: 2009-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9315133 Medline TA: Colloids Surf B Biointerfaces Country: Netherlands |
Other Details:
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Languages: eng Pagination: 238-44 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amines
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chemistry Cyclosporine / pharmacology* Humans Immunosuppressive Agents / chemistry, pharmacology* Liposomes / chemical synthesis, chemistry, pharmacology* Phosphatidylethanolamines / chemistry Phosphoric Acid Esters / chemistry T-Lymphocytes / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Immunosuppressive Agents; 0/Liposomes; 0/Phosphatidylethanolamines; 0/Phosphoric Acid Esters; 124-30-1/stearylamine; 2197-63-9/dicetylphosphate; 2462-63-7/dioleoyl phosphatidylethanolamine; 59865-13-3/Cyclosporine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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