Document Detail


The enhancement of histone H4 and H2A serine 1 phosphorylation during mitosis and S-phase is evolutionarily conserved.
MedLine Citation:
PMID:  15133681     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Histone phosphorylation has long been associated with condensed mitotic chromatin; however, the functional roles of these modifications are not yet understood. Histones H1 and H3 are highly phosphorylated from late G2 through telophase in many organisms, and have been implicated in chromatin condensation and sister chromatid segregation. However, mutational analyses in yeast and biochemical experiments with Xenopus extracts have demonstrated that phosphorylation of H1 and H3 is not essential for such processes. In this study, we investigated additional histone phosphorylation events that may have redundant functions to H1 and H3 phosphorylation during mitosis. We developed an antibody to H4 and H2A that are phosphorylated at their respective serine 1 (S1) residues and found that H4S1/H2AS1 are highly phosphorylated in the mitotic chromatin of worm, fly, and mammals. Mitotic H4/H2A phosphorylation has similar timing and localization as H3 phosphorylation, and closely correlates with the chromatin condensation events during mitosis. We also detected a lower level of H4/H2A phosphorylation in 5-bromo-2-deoxyuridine-positive S-phase cells, which corroborates earlier studies that identified H4S1 phosphorylation on newly synthesized histones during S-phase. The evolutionarily conserved phosphorylation of H4/H2A during the cell cycle suggests that they may have a dual purpose in chromatin condensation during mitosis and histone deposition during S-phase.
Authors:
Cynthia M Barber; Fiona B Turner; Yanming Wang; Kirsten Hagstrom; Sean D Taverna; Sahana Mollah; Beatrix Ueberheide; Barbara J Meyer; Donald F Hunt; Peter Cheung; C David Allis
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2004-05-07
Journal Detail:
Title:  Chromosoma     Volume:  112     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-17     Completed Date:  2005-01-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  360-71     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies / chemistry,  isolation & purification,  metabolism
Chromatin / chemistry,  metabolism
Conserved Sequence
Evolution, Molecular*
Hela Cells
Histones / analysis,  chemistry,  metabolism*
Humans
Interphase
Mitosis*
Molecular Sequence Data
Phosphorylation
Serine / chemistry,  metabolism*
Up-Regulation
Grant Support
ID/Acronym/Agency:
GM37537/GM/NIGMS NIH HHS; GM40922/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Chromatin; 0/Histones; 56-45-1/Serine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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