Document Detail


Enhanced effect of DNA immunization plus in vivo electroporation with a combination of hepatitis B virus core-PreS1 and S-PreS1 plasmids.
MedLine Citation:
PMID:  21900535     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To develop a novel, effective HBV therapeutic vaccine, we constructed two HBV DNA immunogens that contained PreS1, HBSS1, and HBCS1. Several delivery methods, such as intramuscular (i.m.) injection, intramuscular injection plus electroporation (i.m.-EP), and intradermal injection plus electroporation (i.d.-EP) were used in a murine model to analyze and compare the immune responses that were induced by the DNA immunogens. We found that i.d.-EP accelerated specific antibody seroconversion and produced high antibody (anti-PreS1, anti-S, and anti-C antibody) titers after HBSS1 and HBCS1 immunization. Combining the HBSS1 and HBCS1 DNA immunogens with i.d.-EP produced the strongest multiantigen (PreS1, S, and C)-specific cellular immune response and the highest specific PreS1 antibody levels. The results indicated that DNA immunization using HBSS1 and HBCS1 might be an ideal candidate, with its ability to elicit robust B and T cell immune responses against multiantigen when combined with optimized delivery technology. The present study provides a basis for the design and rational application of a novel HBV DNA vaccine.
Authors:
Hong Chen; Bo Wen; Yao Deng; Wen Wang; Xiao Yin; Jie Guan; Li Ruan; Wenjie Tan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-07
Journal Detail:
Title:  Clinical and vaccine immunology : CVI     Volume:  18     ISSN:  1556-679X     ISO Abbreviation:  Clin. Vaccine Immunol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-02     Completed Date:  2012-02-21     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101252125     Medline TA:  Clin Vaccine Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1789-95     Citation Subset:  IM    
Affiliation:
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Yingxin Street, Xuanwu District, Beijing 100052, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocytes / immunology
Electroporation / methods*
Female
Hepatitis B Antibodies / blood
Hepatitis B Surface Antigens / genetics,  immunology*
Hepatitis B Vaccines / administration & dosage*,  genetics,  immunology*
Immunization / methods*
Injections, Intradermal
Injections, Intramuscular
Mice
Mice, Inbred BALB C
Plasmids
T-Lymphocytes / immunology
Vaccines, DNA / administration & dosage*,  genetics,  immunology*
Chemical
Reg. No./Substance:
0/Hepatitis B Antibodies; 0/Hepatitis B Surface Antigens; 0/Hepatitis B Vaccines; 0/Vaccines, DNA
Comments/Corrections

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