| The Mus81/Mms4 endonuclease acts independently of double-Holliday junction resolution to promote a distinct subset of crossovers during meiosis in budding yeast. | |
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MedLine Citation:
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PMID: 12750322 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Current models for meiotic recombination require that crossovers derive from the resolution of a double-Holliday junction (dHJ) intermediate. In prokaryotes, enzymes responsible for HJ resolution are well characterized but the identification of a eukaryotic nuclear HJ resolvase has been elusive. Indirect evidence suggests that MUS81 from humans and fission yeast encodes a HJ resolvase. We provide three lines of evidence that Mus81/Mms4 is not the major meiotic HJ resolvase in S. cerevisiae: (1) MUS81/MMS4 is required to form only a distinct subset of crossovers; (2) rather than accumulating, dHJ intermediates are reduced in an mms4 mutant; and (3) expression of a bacterial HJ resolvase has no suppressive effect on mus81 meiotic phenotypes. Our analysis also reveals the existence of two distinct classes of crossovers in budding yeast. Class I is dependent upon MSH4/MSH5 and exhibits crossover interference, while class II is dependent upon MUS81/MMS4 and exhibits no interference. mms4 specifically reduces crossing over on small chromosomes, which are known to undergo less interference. The correlation between recombination rate and degree of interference to chromosome size may therefore be achieved by modulating the balance between class I/class II crossovers. |
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Authors:
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Teresa de los Santos; Neil Hunter; Cindy Lee; Brittany Larkin; Josef Loidl; Nancy M Hollingsworth |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Genetics Volume: 164 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-05-16 Completed Date: 2004-01-30 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 81-94 Citation Subset: IM |
Affiliation:
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Institute for Cell and Developmental Biology, Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 11794-5215, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Crossing Over, Genetic* DNA, Cruciform DNA-Binding Proteins / genetics, metabolism* Endonucleases* Flap Endonucleases Meiosis / genetics, physiology Saccharomyces cerevisiae / enzymology, genetics* Saccharomyces cerevisiae Proteins* Trans-Activators / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM-50717/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Cruciform; 0/DNA-Binding Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Trans-Activators; EC 3.1.-/Endonucleases; EC 3.1.-/Flap Endonucleases; EC 3.1.-/MUS81 protein, S cerevisiae; EC 3.1.22.-/MMS4 protein, S cerevisiae |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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