| The endocytic penetration mechanism of iron oxide magnetic nanoparticles with positively charged cover: a morphological approach. | |
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MedLine Citation:
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PMID: 20818493 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study we present a morphological approach in observing the interaction of cationic magnetic nanoparticles with A-549 cells (human lung adenocarcinoma). Under our experimental conditions, nanoparticles easily penetrated cells and were observed in vivo, using bright light microscopy. In fixed cells, nanoparticles remained inside cells, showing quantity and distribution patterns similar to those in unfixed cells. The presence of nanoparticles did not affect cell viability or the morphologic parameters assessed. We determined the potential internalization mechanism of nanoparticles into cells using endocytosis inhibitors. The results suggest that nanoparticles used in this study penetrate A-549 cells mainly through a macropinocytosis process. |
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Authors:
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Magdalena Cañete; Jorge Soriano; Angeles Villanueva; Alejandro G Roca; Sabino Veintemillas; Carlos J Serna; Rodolfo Miranda; Maria Del Puerto Morales |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 26 ISSN: 1791-244X ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 533-9 Citation Subset: IM |
Affiliation:
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Departamento de Biología, Universidad Autónoma de Madrid C/ Darwin 2, Cantoblanco, Madrid, Spain. magdalena.canete@uam.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cations
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chemistry,
metabolism Cell Line, Tumor Cell Survival Clathrin / metabolism Endocytosis* Ferric Compounds / chemistry, metabolism* Humans Magnetics Microtubules / metabolism, ultrastructure Nanoparticles / chemistry*, ultrastructure Particle Size Static Electricity Tubulin / analysis |
| Chemical | |
Reg. No./Substance:
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0/Cations; 0/Clathrin; 0/Ferric Compounds; 0/Tubulin; 1309-37-1/ferric oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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