Document Detail

The endocytic penetration mechanism of iron oxide magnetic nanoparticles with positively charged cover: a morphological approach.
MedLine Citation:
PMID:  20818493     Owner:  NLM     Status:  MEDLINE    
In this study we present a morphological approach in observing the interaction of cationic magnetic nanoparticles with A-549 cells (human lung adenocarcinoma). Under our experimental conditions, nanoparticles easily penetrated cells and were observed in vivo, using bright light microscopy. In fixed cells, nanoparticles remained inside cells, showing quantity and distribution patterns similar to those in unfixed cells. The presence of nanoparticles did not affect cell viability or the morphologic parameters assessed. We determined the potential internalization mechanism of nanoparticles into cells using endocytosis inhibitors. The results suggest that nanoparticles used in this study penetrate A-549 cells mainly through a macropinocytosis process.
Magdalena Cañete; Jorge Soriano; Angeles Villanueva; Alejandro G Roca; Sabino Veintemillas; Carlos J Serna; Rodolfo Miranda; Maria Del Puerto Morales
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  26     ISSN:  1791-244X     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-06     Completed Date:  2011-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  533-9     Citation Subset:  IM    
Departamento de Biología, Universidad Autónoma de Madrid C/ Darwin 2, Cantoblanco, Madrid, Spain.
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MeSH Terms
Cations / chemistry,  metabolism
Cell Line, Tumor
Cell Survival
Clathrin / metabolism
Ferric Compounds / chemistry,  metabolism*
Microtubules / metabolism,  ultrastructure
Nanoparticles / chemistry*,  ultrastructure
Particle Size
Static Electricity
Tubulin / analysis
Reg. No./Substance:
0/Cations; 0/Clathrin; 0/Ferric Compounds; 0/Tubulin; 1309-37-1/ferric oxide

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