| The endocannabinoid 2-arachidonoylglycerol (2-AG) and metabolizing enzymes during rat fetoplacental development: a role in uterine remodelling. | |
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MedLine Citation:
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PMID: 20727980 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The main endocannabinoids (EC) identified in mammalian tissues are N-arachidonoylethanolamide (AEA, anandamide), and 2-arachidonoylglycerol (2-AG). AEA levels are critical in pregnancy, especially during implantation, decidualization, and placental development. As 2-AG functions in pregnancy are still largely undefined, we hypothesized that it may also have a role during fetoplacental development. We showed that 2-AG is not only present in the rat mesometrial decidua and plasma during fetoplacental development, but that both 2-AG synthesizing (diacylglycerol lipase) and degradation (monoacylglycerol lipase) enzymes are expressed by decidual cells. While lower concentrations of 2-AG induced apoptosis of rat primary decidual cells, via the CB1 receptor, higher concentrations induced a dramatic effect on cell morphology, cell viability and lactate dehydrogenase release, triggered through a mechanism independent of CB1. This study provides evidences that 2-AG fluctuation in maternal tissues throughout normal pregnancy is primarily regulated by its metabolizing enzymes. Together, these data supports the hypothesis that a deregulation of the endocannabinoid system through aberrant cannabinoid signalling may impact normal uterine remodelling process and consequently normal pregnancy. |
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Authors:
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B M Fonseca; G Correia-da-Silva; A H Taylor; P M W Lam; T H Marczylo; S C Bell; J C Konje; N A Teixeira |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-19 |
Journal Detail:
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Title: The international journal of biochemistry & cell biology Volume: 42 ISSN: 1878-5875 ISO Abbreviation: Int. J. Biochem. Cell Biol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-05 Completed Date: 2011-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508482 Medline TA: Int J Biochem Cell Biol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1884-92 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Departamento de Bioquímica, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / genetics, physiology Arachidonic Acids / metabolism* Blotting, Western Cell Survival Cells, Cultured Decidua / cytology, metabolism Female Glycerides / metabolism* Immunohistochemistry Lipoprotein Lipase / genetics, metabolism Male Monoacylglycerol Lipases / genetics, metabolism Placentation / genetics, physiology* Polymerase Chain Reaction Pregnancy Rats Rats, Wistar Uterus / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Glycerides; 53847-30-6/2-arachidonylglycerol; EC 3.1.1.23/Monoacylglycerol Lipases; EC 3.1.1.34/Lipoprotein Lipase |
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