Document Detail


The emitting species dissociated from the enzyme can emit the light in Photinus pyralis luciferase system.
MedLine Citation:
PMID:  10558856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By fluorescent results, it has been proposed that the product (excited oxyluciferin) bounded in the firefly luciferase emits the light. Also it is generally accepted that two forms (keto and enol) of the oxyluciferin emit red and yellow-green light, respectively. In order to demonstrate the existence of free emitting species using His-tagged luciferase (His-luc), N-terminal 6x His-tagged luciferase (Photinus pyralis) was prepared and expressed in E. coli. After immobilization of His-luc on the membrane by IDA-Ni(2+)-His complex, His-luc clearly showed spectral changes of the emission toward red light. The luciferase-free product obtained from enzymatic reaction mixture in the presence of ATP and dATP emits the light with maximal wavelengths of 575 and 620 nm, respectively. Based on these results, we suppose that two different emitting species or two different energy levels of the emitting species are responsible for two different color lights.
Authors:
K L Min; J P Steghens
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  265     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-20     Completed Date:  1999-12-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  273-8     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
College of Natural Sciences and Research Center for Molecular Microbiology, Seoul National University, Seoul, 151-742, Republic of Korea. kyunglyummin@yahoo.co.kr
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MeSH Terms
Descriptor/Qualifier:
Animals
Beetles / enzymology*,  genetics
Escherichia coli / genetics
Firefly Luciferin / chemistry*,  metabolism
Indoles*
Luciferases / chemistry*,  genetics,  metabolism*
Luminescent Measurements
Membranes, Artificial
Models, Chemical
Nickel / pharmacology
Photochemistry
Pyrazines*
Recombinant Proteins / chemistry,  genetics,  metabolism
Spectrophotometry
Chemical
Reg. No./Substance:
0/Indoles; 0/Membranes, Artificial; 0/Pyrazines; 0/Recombinant Proteins; 17297-78-8/oxyluciferin; 2591-17-5/Firefly Luciferin; 7440-02-0/Nickel; EC 1.13.12.-/Luciferases

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