Document Detail


The embryonic stem cell test.
MedLine Citation:
PMID:  23138917     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The embryonic stem cell test is an animal-free alternative test method for developmental toxicity. Mouse embryonic stem cells are cultured in a hanging drop method to form embryoid bodies. These embryoid bodies, when plated on tissue culture dishes, differentiate to form contracting myocardial cell foci within 10 days. Inhibition of cardiomyocyte differentiation by test compounds serves as the end point of the assay, as monitored by counting contracting muscle foci under the microscope.
Authors:
Sjors H W Schulpen; Aldert H Piersma
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  947     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  375-82     Citation Subset:  IM    
Affiliation:
Laboratory for Health Protection Research-National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands, sjors.schulpen@rivm.nl.
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