Document Detail


emb-4 is a conserved gene required for efficient germline-specific chromatin remodeling during Caenorhabditis elegans embryogenesis.
MedLine Citation:
PMID:  17028322     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In C. elegans, germline blastomeres are initially kept transcriptionally quiescent by the maternally loaded CCCH zinc-finger protein PIE-1. PIE-1 disappears upon the birth of the primordial germ cells Z2 and Z3, yet these cells appear to remain quiescent. We have previously demonstrated that there is a chromatin-based repression that succeeds PIE-1 degradation. The chromatin in Z2/Z3 loses certain histone modifications, including histone H3 lysine 4 dimethylation (H3K4me2), a conserved marker for transcriptionally competent chromatin. We find that mutations in the maternal-effect gene emb-4 cause defects in both PIE-1 degradation and germline-specific chromatin remodeling. emb-4 encodes a highly conserved protein with orthologs in fly, mouse, and human and has a subtle role in Notch signaling. The embryonic phenotype of emb-4 is consistent with a defect in the efficient and timely activation of developmental programs, including germline chromatin remodeling. We also find that, as in early somatic blastomeres, the degradation of PIE-1 in Z2/Z3 is facilitated by zinc-finger-interacting protein ZIF-1, and in the absence of either zif-1 or emb-4, PIE-1 is abnormally retained in Z2/Z3.
Authors:
Paula M Checchi; William G Kelly
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-10-08
Journal Detail:
Title:  Genetics     Volume:  174     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-21     Completed Date:  2007-02-20     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1895-906     Citation Subset:  IM    
Affiliation:
Biology Department, Emory University, Atlanta, Georgia 30322, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / cytology,  embryology,  genetics*
Caenorhabditis elegans Proteins / genetics,  metabolism
Carrier Proteins / genetics,  metabolism
Cell Lineage*
Chromatin Assembly and Disassembly*
Embryo, Nonmammalian / cytology,  metabolism*
Embryonic Development
Gene Expression Regulation, Developmental
Germ Cells / physiology*
Histones / metabolism
Mutation / genetics
Nuclear Proteins / genetics,  metabolism
Phenotype
Grant Support
ID/Acronym/Agency:
GM077600/GM/NIGMS NIH HHS; GM63102/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Carrier Proteins; 0/Histones; 0/Nuclear Proteins; 0/ZIF-1 protein, C elegans; 0/pie-1 protein, C elegans
Comments/Corrections

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