Document Detail

p21WAF1/CIP1/SDI1 is elevated through a p53-independent pathway by mimosine.
MedLine Citation:
PMID:  8809407     Owner:  NLM     Status:  MEDLINE    
Tumor suppressor p53 is a nuclear protein that is induced by DNA damage and is involved in G1 and G2 phase control of the cell cycle. p21WAF1/CIP1/SDI1 (p21), a cyclin-dependent kinase inhibitor, is a downstream target and effector of p53 to induce G1 arrest. Mimosine is a potent reversible late G1 phase blocker of the cell cycle. In this study, we showed that mimosine can increase both p21 mRNA and protein levels, indirectly inhibit cyclin E-associated kinase activity without affecting the cyclin E protein level, block human breast cancer cells (21PT) in the late G1 phase of the cell cycle, and induce a p53-independent p21 pathway in these cells. These results support the possibility of restoring a G1 checkpoint by use of mimosine. They also suggest that the mechanism of the effect of mimosine is complex and may have more than one target in the cell.
R S Alpan; A B Pardee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  7     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1997-04-29     Completed Date:  1997-04-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  893-901     Citation Subset:  IM    
Division of Cell Growth and Regulation, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
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MeSH Terms
Breast Neoplasms / metabolism,  pathology
Cell Cycle / drug effects*
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / metabolism*
Mimosine / pharmacology*
RNA, Messenger / biosynthesis
Signal Transduction / drug effects*
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / metabolism*
Grant Support
5-R01 GM24571-15/GM/NIGMS NIH HHS
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53; 500-44-7/Mimosine

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