Document Detail


On the electrophysiological response of bone cells using a Stokesian fluid stimulus probe for delivery of quantifiable localized picoNewton level forces.
MedLine Citation:
PMID:  21511259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A Stokesian fluid stimulus probe (SFSP), capable of delivering quantifiable pN level hydrodynamic forces, is developed to distinguish the electrophysiological response of the cell process and cell body of osteocyte-like MLO-Y4 cells without touching the cell or its substrate. The hydrodynamic disturbance is a short lived (100 ms), constant strength pressure pulse that propagates nearly instantaneously through the medium creating a nearly spherical expanding fluid bolus surrounding a 0.8 μm micropipette tip. Laboratory model experiments show that the growth of the bolus and the pressure field can be closely modeled by quasi-steady Stokes flow through a circular orifice provided the tip Reynolds number, Re(t)<0.03. By measuring the deflection of the dendritic processes between discrete attachment sites, and applying a detailed ultrastructural model for the central actin filament bundle within the process, one is able to calculate the forces produced by the probe using elastic beam theory. One finds that forces between 1 and 2.3 pN are sufficient to initiate electrical signaling when applied to the cell process, but not the much softer cell body. Even more significantly, cellular excitation by the process only occurs when the probe is directed at discrete focal attachment sites along the cell process. This suggests that electrical signaling is initiated at discrete focal attachments along the cell process and that these sites are likely integrin-mediated complexes associated with stretch-activated ion channels though their molecular structure is unknown.
Authors:
Danielle Wu; Peter Ganatos; David C Spray; Sheldon Weinbaum
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-04-20
Journal Detail:
Title:  Journal of biomechanics     Volume:  44     ISSN:  1873-2380     ISO Abbreviation:  J Biomech     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-23     Completed Date:  2011-10-03     Revised Date:  2013-08-01    
Medline Journal Info:
Nlm Unique ID:  0157375     Medline TA:  J Biomech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1702-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biomedical Engineering, The City College of New York, Steinman Hall, Room T-404B, Convent Avenue and 140th Street, New York, NY 10031, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / chemistry
Bone and Bones / physiology
Electrochemistry / methods
Electrophysiology / methods*
Equipment Design
Humans
Hydrodynamics
Materials Testing
Neurons / physiology*
Osteocytes / cytology
Patch-Clamp Techniques
Pressure
Signal Processing, Computer-Assisted
Signal Transduction
Stress, Mechanical
Grant Support
ID/Acronym/Agency:
AR057139/AR/NIAMS NIH HHS; R01 AR057139/AR/NIAMS NIH HHS; R01 AR057139-01A2/AR/NIAMS NIH HHS; R01 AR057139-02/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Actins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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