| An electrophoretic method for the determination of the proportion of gamma-aminobutyric acid in a mixture of labeled neurotransmitter and its catabolites. | |
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MedLine Citation:
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PMID: 2882707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An electrophoretic method for the separation of gamma-aminobutyric acid (GABA) from its metabolites after GABA-transaminase attack is presented. The method is based on the fact that at neutral pH GABA has no net electrical charge, whereas its major metabolites, succinic acid and Krebs cycle intermediates, are negatively charged. The method appears to be especially suitable for evaluation of true-labeled neurotransmitter within the radioactivity which is found in synaptosomes after labeled GABA-uptake studies. |
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Authors:
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A Cupello; M V Rapallino; G Besio; P Mainardi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 160 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1987 Jan |
Date Detail:
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Created Date: 1987-05-20 Completed Date: 1987-05-20 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 14-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4-Aminobutyrate Transaminase Animals Brain / metabolism Carbon Radioisotopes Chromatography, Thin Layer / methods Electrophoresis, Agar Gel / methods Neurotransmitter Agents / analysis* Radioisotope Dilution Technique Rats Synaptosomes / metabolism gamma-Aminobutyric Acid / analysis*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Neurotransmitter Agents; 56-12-2/gamma-Aminobutyric Acid; EC 2.6.1.19/4-Aminobutyrate Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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