| The electrokinetic behavior of red blood cells from a patient with Tn syndrome by Doppler electrophoretic light scattering analysis. | |
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MedLine Citation:
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PMID: 7732519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Tn syndrome is an acquired form of persistent mixed-field polyagglutination displaying two distinct populations of Tn positive (Tn) and Tn negative (tn) red blood cells (RBCs). We investigated the electrophoretic behavior of RBCs showing polyagglutination from a patient with Tn syndrome by the doppler electrophoretic light scattering (D.E.L.S.) analysis. The mean of zeta potential of normal RBCs from ten individuals was -13.07 +/- 0.61 mV (mean +/- S.D.). The content of membrane-associated sialic acid equated with the zeta potential of RBCs. Among the proteases ficin was most effective on the zeta potential of RBCs. The zeta potential of the patient Tn RBCs and tn RBCs were -4.73 mV and -13.32 mV, respectively. Tn RBCs reduced 64.5% of zeta potential compared with tn RBCs and formed 48.8%. These results may provide some useful information for classification of Tn syndrome. |
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Authors:
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T Omi; E Kajii; S Ikemoto |
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Publication Detail:
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Type: Case Reports; Journal Article |
Journal Detail:
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Title: The Tohoku journal of experimental medicine Volume: 174 ISSN: 0040-8727 ISO Abbreviation: Tohoku J. Exp. Med. Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1995-05-30 Completed Date: 1995-05-30 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0417355 Medline TA: Tohoku J Exp Med Country: JAPAN |
Other Details:
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Languages: eng Pagination: 369-77 Citation Subset: IM |
Affiliation:
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Department of Legal Medicine and Human Genetics, Jichi Medical School, Tochigi, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Tumor-Associated, Carbohydrate* Doppler Effect Electrophoresis Enzymes / pharmacology Erythrocyte Membrane / chemistry, drug effects Erythrocytes* / drug effects Hemagglutination* Hematologic Diseases / blood* Humans Light Male Middle Aged N-Acetylneuraminic Acid Scattering, Radiation Sialic Acids / chemistry Surface Properties Syndrome |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Tumor-Associated, Carbohydrate; 0/Enzymes; 0/Sialic Acids; 0/Tn antigen; 131-48-6/N-Acetylneuraminic Acid |
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