Document Detail


An electrochemical impedimetric immunosensor for label-free detection of Campylobacter jejuni in diarrhea patients' stool based on O-carboxymethylchitosan surface modified Fe3O4 nanoparticles.
MedLine Citation:
PMID:  19932018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel electrochemical impedimetric immunosensor based on O-carboxymethylchitosan surface modified Fe(3)O(4) nanoparticles (denoted as OCMCS-Fe(3)O(4) nanoparticles) was developed for rapid detection of Campylobacter jejuni, which is becoming the most common cause of gastroenteritis in developed countries and raising major public health concerns worldwide. In the present study, anti-FlaA monoclonal antibodies 2D12 (denoted as 2D12McAbs) were immobilized on OCMCS-Fe(3)O(4) nanoparticles. The detection was performed by measuring relative change in impedance before and after 2D12McAbs-Campylobacter jejuni reaction with the technique of electrochemical impedance spectroscopy. Under the optimized conditions, the relative change in impedance was proportional to the logarithmic value of Campylobacter jejuni concentrations in the range of 1.0x10(3) to 1.0x10(7) CFU/mL (r=0.991). The advantages of the OCMCS-Fe(3)O(4) nanoparticle-based immunosensor are simplicity of use, fast response, wide linear range, acceptable reproducibility and long stability. Moreover, the immunosensor could be regenerated by being treated with glycine-HCl buffer solution (pH 2.8). We demonstrate the convenient application of the novel immunosensor for the detection of Campylobacter jejuni in diarrhea patients' stool samples.
Authors:
Jinlin Huang; Gongjun Yang; Wenjing Meng; Liping Wu; Aiping Zhu; Xin'an Jiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-04
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  25     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1204-11     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Affiliation:
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques / instrumentation
Campylobacter jejuni / isolation & purification*
Diarrhea / diagnosis,  microbiology*
Electric Impedance
Electrochemistry / instrumentation*
Equipment Design
Equipment Failure Analysis
Feces / microbiology*
Ferric Compounds / chemistry*
Humans
Immunoassay / instrumentation*
Nanoparticles / chemistry*
Reproducibility of Results
Sensitivity and Specificity
Surface Properties
Chemical
Reg. No./Substance:
0/Ferric Compounds; 1309-37-1/ferric oxide

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