Document Detail


The electrochemical H+ gradient in the yeast Rhodotorula glutinis.
MedLine Citation:
PMID:  4040135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The electrochemical gradient of protons, delta mu H+, was estimated in the obligatory aerobic yeast Rhodotorula glutinis in the pH0 range from 3 to 8.5. The membrane potential, delta psi, was measured by steady-state distribution of the hydrophobic ions, tetraphenylphosphonium (TPP+) for negative delta psi above pH0 4.5, and thiocyanate (SCN-) for positive delta psi below pH0 4.5. The chemical gradient of H+ was determined by measuring the chemical shift of intracellular Pi by 31P-NMR at given pH0 values. The values of pHi increased almost linearly from 7.3 at pH0 3 to 7.8 at pH0 8.5. In the physiological pH0 range from 3.5 to 6, delta mu H+ was fairly constant at values between 17-18 KJ mol-1, gradually decreasing at pH0 above 6. In deenergized cells, the intracellular pHi decreased to values as low as 6, regardless of whether the cell suspension was buffered at pH0 4.5 or 7.5. There was no membrane potential detectable in deenergized cells.
Authors:
M Höfer; K Nicolay; G Robillard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bioenergetics and biomembranes     Volume:  17     ISSN:  0145-479X     ISO Abbreviation:  J. Bioenerg. Biomembr.     Publication Date:  1985 Jun 
Date Detail:
Created Date:  1985-08-06     Completed Date:  1985-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7701859     Medline TA:  J Bioenerg Biomembr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  175-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
Electrochemistry*
Electron Transport
Hydrogen-Ion Concentration*
Intracellular Fluid / analysis
Magnetic Resonance Spectroscopy
Membrane Potentials
Mitosporic Fungi / physiology*
Nitriles / pharmacology
Onium Compounds
Organophosphorus Compounds
Rhodotorula / physiology*
Thiocyanates
Chemical
Reg. No./Substance:
0/Nitriles; 0/Onium Compounds; 0/Organophosphorus Compounds; 0/Thiocyanates; 18198-39-5/tetraphenylphosphonium; 302-04-5/thiocyanate; 51601-81-1/SF 6847; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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