Document Detail

The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegans embryo.
MedLine Citation:
PMID:  17042944     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Fertilization restores the diploid state and begins the process by which the single-cell oocyte is converted into a polarized, multicellular organism. In the nematode, Caenorhabditis elegans, two of the earliest events following fertilization are secretion of the chitinous eggshell and completion of meiosis, and in this report we demonstrate that the eggshell is essential for multiple developmental events at the one-cell stage. RESULTS: We show that the GLD (Germline differentiation abnormal)-1-regulated hexosamine pathway enzyme, glucosamine-6-phosphate N-acetyltransferase (GNA)-2, is required for synthesis of uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), the substrate for eggshell chitin synthesis by chitin synthase-1 (CHS-1). Furthermore, while chs-1(RNAi) or combined RNAi with the chitin-binding proteins, CEJ-1 and B0280.5, does not interfere with normal meiotic timing, lagging chromosomes are observed at meiosis, and polar-body extrusion fails. We also demonstrate that chitin, and either CEJ-1 or B0280.5, are essential for the osmotic/permeability barrier and for movement of the sperm pronucleus/centrosome complex to the cortex, which is associated with the initiation of polarization. CONCLUSION: Our results indicate that the eggshell is required in single-cell C. elegans development, playing an essential role in multiple actin-dependent early events. Furthermore, the earliest meiotic roles precede osmotic barrier formation, indicating that the role of the eggshell is not limited to generation of the osmotic barrier.
Wendy L Johnston; Aldis Krizus; James W Dennis
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-10-16
Journal Detail:
Title:  BMC biology     Volume:  4     ISSN:  1741-7007     ISO Abbreviation:  BMC Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-11-08     Completed Date:  2007-01-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101190720     Medline TA:  BMC Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  35     Citation Subset:  IM    
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Ave, Toronto, ON, M5G 1X5, Canada.
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MeSH Terms
Caenorhabditis elegans / cytology*,  embryology*,  physiology
Egg Shell / cytology*,  embryology*,  physiology
Embryo, Nonmammalian / cytology*,  embryology*,  physiology
Meiosis / physiology*
Ovum / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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