| An efficient surface modification using 2-methacryloyloxyethyl phosphorylcholine to control cell attachment via photochemical reaction in a microchannel. | |
| | |
MedLine Citation:
|
PMID: 20498909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This report describes a direct approach for cell micropatterning in a closed glass microchannel. To control the cell adhesiveness inside the microchannel, the application of an external stimulus such as ultraviolet (UV) was indispensible. This technique focused on the use of a modified 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, which is known to be a non-biofouling compound that is a photocleavable linker (PL), to localize cells via connection to an amino-terminated silanized surface. Using UV light illumination, the MPC polymer was selectively eliminated by photochemical reaction that controlled the cell attachment inside the microchannel. For suitable cell micropatterning in a microchannel, the optimal UV illumination time and concentration for cell suspension were investigated. After selective removal of the MPC polymer through the photomask, MC-3T3 E1 cells and vascular endothelial cells (ECs) were localized only to the UV-exposed area. In addition, the stability of patterned ECs was also confirmed by culturing for 2 weeks in a microchannel under flow conditions. Furthermore, we employed two different types of cells inside the same microchannel through multiple removal of the MPC polymer. ECs and Piccells were localized in both the upper and down streams of the microchannel, respectively. When the ECs were stimulated by adenosine triphosphate (ATP), NO was secreted from the ECs and could be detected by fluorescence resonance energy transfer (FRET) in Piccells, which is a cell-based NO indicator. This technique can be a powerful tool for analyzing cell interaction research. |
| | |
Authors:
|
Kihoon Jang; Kae Sato; Yo Tanaka; Yan Xu; Moritoshi Sato; Takahiro Nakajima; Kazuma Mawatari; Tomohiro Konno; Kazuhiko Ishihara; Takehiko Kitamori |
Related Documents
:
|
21443759 - Mucus cells in the digestive tract in prenatal platyfish larvae (poeciliidae, teleostei). 7020929 - Flow cytometry studies of intracellular adriamycin in single cells in vitro. 20862249 - In situ spatiotemporal mapping of flow fields around seeded stem cells at the subcellul... 12776929 - A fluorescence microplate assay using yopro-1 to measure apoptosis: application to hl60... 1401949 - A simple fluorescence method for surface antigen phenotyping of lymphocytes undergoing ... 15228369 - Asymmetry between sister cells in a cancer cell line revealed by chemical cytometry. 9461089 - Localization of inositol 1,4,5-trisphosphate receptors in the rat kidney. 8187979 - Morulae at compaction and the pattern of protein synthesis in mouse embryos. 1550589 - Topogenesis of carboxylesterases: a rat liver isoenzyme ending in -hteht-cooh is a secr... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-25 |
Journal Detail:
|
Title: Lab on a chip Volume: 10 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-07-14 Completed Date: 2010-09-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
|
Languages: eng Pagination: 1937-45 Citation Subset: IM |
Affiliation:
|
Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
pharmacology Animals Cell Adhesion / radiation effects Cell Line Endothelial Cells / cytology*, metabolism* Methacrylates / chemistry* Mice Microfluidic Analytical Techniques / instrumentation, methods* Nitric Oxide / analysis, metabolism Phosphorylcholine / analogs & derivatives*, chemistry Photochemical Processes* Surface Properties / radiation effects Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
|
0/Methacrylates; 10102-43-9/Nitric Oxide; 107-73-3/Phosphorylcholine; 56-65-5/Adenosine Triphosphate; 67881-98-5/2-methacryloyloxyethyl phosphorylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Operando study of iridium acetylacetonate decomposition on amorphous silica-alumina for bifunctional...
Next Document: Isotopic effects on stereodynamics for the two reactions: H + LiH(+)(v = 0, j = 0) --> H(2) + Li(...