| An efficient preparation of polyanionic affinity agent and its evaluation for the measurement of glycated hemoglobin. | |
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MedLine Citation:
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PMID: 15848760 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An efficient method was developed for the preparation of polyanionic affinity agent (3), a key component in the measurement of glycated hemoglobin (GHb). Glycated hemoglobin is an important clinical marker for diagnosis of patients with diabetes and useful to monitor the management of disease. The affinity agent (3) was prepared based on coupling reaction between poly(acrylic acid) (1) and 3-aminophenylboronic acid (2) in water. The critical features of this polymeric affinity agent (3), such as size, boronic acid incorporation ratio and concentration, on the measurement of glycated hemoglobin were evaluated. It was found that the agent (3) prepared using poly(acrylic acid) (1) with 225 kDa molecular weight gave optimal GHb measurement. The performance test results demonstrated that the boronic acid incorporation ratio and concentration of affinity agent (3) play a critical role in the assay and determines the precision of glycated hemoglobin measurement. |
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Authors:
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Rajarathnam E Reddy; You Pan; Donald D Johnson; Yon-Yih Chen; Saul A Datwyler; Michelle S Hauptman; John K Thottathil |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioorganic & medicinal chemistry Volume: 13 ISSN: 0968-0896 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-04-25 Completed Date: 2005-09-08 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 3467-73 Citation Subset: IM |
Affiliation:
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Core R&D Chemistry Group, (Dept: 09MD, Bldg: AP20), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064-6016, USA. rajarathnam.reddy@abbott.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins
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chemical synthesis*,
chemistry,
metabolism Boronic Acids / chemical synthesis*, chemistry, metabolism Chromatography, Affinity Evaluation Studies as Topic Hemoglobin A, Glycosylated / analysis*, metabolism* Humans |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Boronic Acids; 0/Hemoglobin A, Glycosylated; 30418-59-8/3-aminobenzeneboronic acid; 9003-01-4/carbopol 940 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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