Document Detail

An efficient method for extraction of astaxanthin from green alga Haematococcus pluvialis.
MedLine Citation:
PMID:  17002425     Owner:  NLM     Status:  MEDLINE    
Haematococcus pluvialis is one of the potent organisms for production of astaxanthin, a high value ketocarotenoid. Astaxanthin is accumulated in thick-walled cyst cells of Haematococcus. The thick cell wall is made up of sporopollenin-like material, algaenan, which hinders solvent extraction of astaxanthin. In the present study, an improved method for extraction of astaxanthin without homogenization of cells is reported. Extractability of astaxanthin from cyst cells was evaluated by treating cells with various solvents and pretreating the cells with organic and mineral acids at 70 degrees C followed by acetone extraction. Hydrochloric acid treatment facilitated 86-94% extractability of astaxanthin. Treatment time, temperature, and concentration of the acid were found to be critical factors for maximum extractability. The treatment did not affect the astaxanthin ester profile and the treated cells can be preserved until further use.
R Sarada; R Vidhyavathi; D Usha; G A Ravishankar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-27     Completed Date:  2006-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7585-8     Citation Subset:  IM    
Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore 570 020, India.
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MeSH Terms
Algae, Green / chemistry*
Carotenoids / analysis,  isolation & purification
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Hydrochloric Acid
Xanthophylls / isolation & purification
Reg. No./Substance:
0/Xanthophylls; 36-88-4/Carotenoids; 472-61-7/astaxanthine; 67-64-1/Acetone; 7647-01-0/Hydrochloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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