Document Detail

An efficient method for the cryopreservation of fetal human liver hematopoeitic progenitor cells.
MedLine Citation:
PMID:  11359946     Owner:  NLM     Status:  MEDLINE    
The use of human hematopoietic progenitor cells (HPC) for transplantation requires efficient recovery methods and cryopreservation procedures. The purpose of this study was to determine cryopreservation techniques for fetal human liver (FHL) CD34(+) cells. We assessed FHL HPC recovery efficiency after freezing and thawing by viability testing, fluorescence-activated cell sorting analysis, and colony-forming ability under different conditions. We also determined optimal cell freezing concentrations and the effect of rate-controlled freezing on cell recovery. Lastly, cell recovery after varying freezing time periods was examined. Our results indicated that optimal cell recovery occurs when: A) cryopreservation medium consists of either 5% dimethylsulphoxide (DMSO) or 10% DMSO in combination with either 20% fetal bovine serum (FBS) or 70% FBS and when Iscove's modified Dulbecco's medium consists of not more than 10% DMSO; B) a rate-controlled freezing device container is used; C) CD34(+) cells are frozen at a concentration of 1 x 10(6)/ml, and D) a thawing temperature of 37 degrees C is used. These observations indicate that cryopreservation of FHL HPC is possible for up to 18 months in optimal conditions without losing hematopoietic activity.
J Zhao; H N Hao; R L Thomas; W D Lyman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  19     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2001  
Date Detail:
Created Date:  2001-05-21     Completed Date:  2001-07-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  212-8     Citation Subset:  IM    
Children's Research Center of Michigan, USA.
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MeSH Terms
Antigens, CD34 / biosynthesis
Cell Culture Techniques / methods*
Cell Separation
Cryopreservation / methods*
Dimethyl Sulfoxide / pharmacology
Flow Cytometry
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells / cytology*
Liver / cytology*,  embryology
Time Factors
Reg. No./Substance:
0/Antigens, CD34; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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