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An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats.
MedLine Citation:
PMID:  25049469     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
An efficient protocol was developed for the rapid in vitro multiplication of an endemic and critically endangered medicinal herb, Ceropegia noorjahaniae Ans., via enhanced axillary bud proliferation from nodal explants. The effects of phytohormones [6-benzylaminopurine (BAP), kinetin (Kin) thidiazuron (TDZ), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA)] on in vitro regeneration were investigated. The highest number of shoots (18.3 ± 1.3), maximum shoot length (10.1 ± 0.8 cm) and the highest response of shoot induction (95 %) were recorded on MS medium supplemented with 2.0 mg/l BAP. Rooting was best achieved on half-strength MS medium augmented with IBA (1.0 mg/l). Half-strength MS medium supplemented with BAP (4 mg/l) and sucrose (5 %, w/v) produced an average of 5.6 flower buds per microshoots with highest (90 %) flower bud induction response. The plantlets regenerated in vitro with well-developed shoot and roots were successfully established in pots containing sterile sand and coco peat (1:1) and grown in a greenhouse with 85 % survival rate. The regenerated plants did not show any detectable morphological variation. The developed method can be successfully employed for large-scale multiplication and conservation of C. noorjahaniae.
Authors:
J J Chavan; A S Nalawade; N B Gaikwad; R V Gurav; G B Dixit; S R Yadav
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Publication Detail:
Type:  Journal Article     Date:  2014-06-07
Journal Detail:
Title:  Physiology and molecular biology of plants : an international journal of functional plant biology     Volume:  20     ISSN:  0971-5894     ISO Abbreviation:  Physiol Mol Biol Plants     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-07-22     Completed Date:  2014-07-22     Revised Date:  2014-07-24    
Medline Journal Info:
Nlm Unique ID:  101304333     Medline TA:  Physiol Mol Biol Plants     Country:  India    
Other Details:
Languages:  eng     Pagination:  405-10     Citation Subset:  -    
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