Document Detail


An efficient grafting technique for recovery of transgenic cotton plants.
MedLine Citation:
PMID:  23143491     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Recovery of transgenic cotton plants from tissue culture condition to greenhouse condition is a critical step for improving cotton through genetic engineering. Traditional methods always cause low survival rate of transplanted plants. In 1998, we developed an efficient grafting technique for recovery of transgenic cotton plants, which significantly increased the survival rate of the transplanting regeneration plants. In this chapter, we present a detailed protocol for grafting transgenic cotton plants obtaining somatic embryogenesis.
Authors:
Min Wang; Qinglian Wang; Baohong Zhang
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  958     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  161-4     Citation Subset:  IM    
Affiliation:
Beijing Key Laboratory of Plant Resources Research and Development, Department of Biotechnology, School of Science, Beijing Technology and Business University, Haidian District, Beijing, People's Republic of China.
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