Document Detail

The efficiency of nonsense-mediated mRNA decay is an inherent character and varies among different cells.
MedLine Citation:
PMID:  17625509     Owner:  NLM     Status:  MEDLINE    
Nonsense-mediated mRNA decay (NMD) is a mechanism, which selectively degrades transcripts carrying premature termination codons (PTCs) and a variety of physiologic transcripts containing NMD-inducing features. In a recent study, we have found variable NMD efficiency among nasal epithelial cells obtained from cystic fibrosis (CF) patients. This variability was found for CF transmembrane conductance regulator (CFTR) transcripts carrying the W1282X PTC, as well as for several NMD physiologic substrates. Here, we aimed to investigate the possibility that variability in NMD efficiency is a more generalized phenomenon and is not restricted to nasal epithelial cells. To investigate this possibility, we analyzed the NMD efficiency of both a CFTR constructs carrying the W1282X PTC and beta-globin constructs carrying the NS39 PTC, in HeLa and MCF7 cells. Variability in NMD efficiency was found for both constructs between the cells, such that in HeLa cells the NMD was highly efficient and in MCF7 the efficiency was significantly lower. Moreover, similar differences in the efficiency of NMD were found for five endogenous NMD physiologic transcripts. Altogether, our results demonstrate existence of cells in which NMD of all transcripts is efficient, whereas others in which the NMD is less efficient, suggesting that the efficiency of NMD is an inherent character of cells. Our results also suggest that variability in the efficiency of NMD is a general phenomenon and is not restricted to nasal epithelial cells. As NMD affects the level of many transcripts, variability in the NMD efficiency might play a role as a genetic modifier of different cellular functions.
Liat Linde; Stephanie Boelz; Gabriele Neu-Yilik; Andreas E Kulozik; Batsheva Kerem
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-11
Journal Detail:
Title:  European journal of human genetics : EJHG     Volume:  15     ISSN:  1018-4813     ISO Abbreviation:  Eur. J. Hum. Genet.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-25     Completed Date:  2008-01-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9302235     Medline TA:  Eur J Hum Genet     Country:  England    
Other Details:
Languages:  eng     Pagination:  1156-62     Citation Subset:  IM    
Department of Genetics, Life Sciences Institute, The Hebrew University, Jerusalem, Israel.
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MeSH Terms
Cell Line, Tumor
Codon, Nonsense / physiology*
Genetic Variation
Hela Cells
RNA Stability / physiology*
RNA, Messenger / metabolism*
Reg. No./Substance:
0/Codon, Nonsense; 0/RNA, Messenger

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