| The effects of zoledronic acid on neointimal hyperplasia: a rabbit carotid anastomosis model. | |
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MedLine Citation:
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PMID: 21285021 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The aim of the present study was to investigate the effect of zoledronic acid (ZA), as a matrix metalloproteinase inhibitor, on neointimal hyperplasia in rabbit carotid anastomosis model. METHODS: New Zealand male rabbits were divided into two groups as placebo and treatment groups in this experimental study. After anesthesia, the right carotid artery of each rabbit was end-to-end anastomosed with an 8/0 polypropylene suture. Left carotid artery was kept as control without any operation. Placebo group (n=6) received phosphate buffered saline (PBS) (0.5mL/kg/day/s.c.) for 28 days postoperatively, whereas ZA group (n=6) received ZA (100 µg/kg/day/s.c.) for the same period. After sacrification, the anastomosed and control arteries were isolated. Morphometric and immunohistochemical examinations were performed. Statistical analyses of morphometric and immunohistochemical data were performed using two-way ANOVA and Chi-square test respectively. RESULTS: In PBS group, vascular injury in the anastomosed artery significantly increased the intimal area (anastomosed: 112.51±61.18 µm(2)*1000 vs. control: 22.62±4.26 µm(2)*1000, p<0.01) and intima/media index (anastomosed: 0.347±0.29 vs. control: 0.075±0.01, p<0.05) compared to control artery. ZA significantly reduced the intimal area (39.29±18.21 µm(2)*1000 , p<0.01) and intima/media index (0.112±0.07, p<0.05) compared to PBS group. Additionally, α-smooth muscle actin immunopositivity was found significantly decreased in anastomosed arteries from ZA group (ZA: 2.33±0.52 vs. PBS: 3.50±0.5, p<0.05). Moreover, intensive gelatinase (MMP-2 and MMP-9) immunoreactivities were clearly seen in anastomosed arteries compared to control arteries from PBS group. ZA apparently decreased immunopositivities for gelatinases in anastomosed arteries. CONCLUSION: ZA might be a promising agent for prevention of neointimal hyperplasia, which is the most significant cause of graft failures in late postoperative period. |
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Authors:
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Mehmet Güzeloğlu; Mehmet Gül; Buket Reel; Ismail Yürekli; Koray Aykut; Eyüp Hazan |
Publication Detail:
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Type: Journal Article Date: 2011-02-02 |
Journal Detail:
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Title: Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology Volume: 11 ISSN: 1308-0032 ISO Abbreviation: Anadolu Kardiyol Derg Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-07 Completed Date: 2012-01-05 Revised Date: 2012-01-19 |
Medline Journal Info:
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Nlm Unique ID: 101095069 Medline TA: Anadolu Kardiyol Derg Country: Turkey |
Other Details:
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Languages: eng Pagination: 93-100 Citation Subset: IM |
Affiliation:
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Department of Cardiovascular Surgery, Faculty of Medicine, Mustafa Kemal University, Hatay, Turkey. mehmetguzeloglu@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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analysis Anastomosis, Surgical Animals Carotid Arteries / pathology, surgery* Diphosphonates / pharmacology, therapeutic use* Disease Models, Animal Hyperplasia / drug therapy, prevention & control Imidazoles / pharmacology, therapeutic use* Immunohistochemistry Male Matrix Metalloproteinase 2 / analysis Matrix Metalloproteinase 9 / analysis Matrix Metalloproteinases / analysis, antagonists & inhibitors* Muscle, Smooth, Vascular / chemistry, enzymology, pathology Neointima / drug therapy, pathology, prevention & control* Rabbits Random Allocation |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Diphosphonates; 0/Imidazoles; 118072-93-8/zoledronic acid; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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