| The effects of vitamin a compounds on hyaluronic Acid released from cultured rabbit corneal epithelial cells and keratocytes. | |
| | |
MedLine Citation:
|
PMID: 23132305 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
A role of vitamin A in the synthesis of hyaluronic acid by skin cells is well known. Hyaluronic acid is produced by corneal epithelial cells and keratocytes in the eye. We investigated whether rabbit corneal epithelial cells and keratocytes release hyaluronic acid after exposure to vitamin A compounds. Rabbit corneal epithelial cells and keratocytes were inoculated with RCGM2 medium and incubated at 37ºC under 5% CO(2) in air for 24 h. The medium was then replaced with medium containing 0.1, 1, 10, or 100 μM retinoic acid or retinol palmitate (VApal) and incubated for another 48 h. Hyaluronic acid release from both corneal epithelial cells and keratocytes during culture was increased by retinoic acid at the lower concentration of 0.1 μM and 1 μM determined with a sandwich binding protein assay kit. However, it was significantly decreased at the higher concentrations of 10 μM and 100 μM, and the cell count determined with a Neutral Red assay kit was also decreased at these concentrations. On the other hand, hyaluronic acid release from corneal epithelial cells during culture was increased by VApal at the lower concentration of 0.1 μM and 1 μM, but there was no significant difference in the cell count for either corneal epithelial cells or keratocytes in the presence of VApal at any concentration. In conclusion, it is suggested that vitamin A stimulates the release of hyaluronic acid from cultured rabbit corneal epithelial cells and keratocytes. |
| | |
Authors:
|
Hiroshi Toshida; Nobuhito Tabuchi; Daisuke Koike; Misao Koide; Keikichi Sugiyama; Kiyoo Nakayasu; Atsushi Kanai; Akira Murakami |
Related Documents
:
|
9734865 - Urinary and biliary excretion of ergot alkaloids from steers that grazed endophyte-infe... 525335 - The excretion of c6-c10-dicarboxylic acids in the urine of newborn infants during starv... 9283125 - Serum and urinary amino acid levels in sickle cell disease. 19891605 - Health consequences of catabolic synthesis of hippuric acid in humans. 8881715 - Modification of the fatty acid composition of dietary oils and fats on incorporation in... 102085 - An improved method for gas chromatographic determination of urinary xylitol and glucuro... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of nutritional science and vitaminology Volume: 58 ISSN: 1881-7742 ISO Abbreviation: J. Nutr. Sci. Vitaminol. Publication Date: 2012 |
Date Detail:
|
Created Date: 2012-11-07 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0402640 Medline TA: J Nutr Sci Vitaminol (Tokyo) Country: Japan |
Other Details:
|
Languages: eng Pagination: 223-9 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology, Juntendo University Shizuoka Hospital. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by...
Next Document: The Interactive Effect of Dietary Water-Soluble Vitamin Levels on the Depression of Gonadal Developm...