| The effects of vascular endothelial growth factor C knockdown in esophageal squamous cell carcinoma. | |
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MedLine Citation:
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PMID: 22057733 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE: We investigated the role of vascular endothelial growth factor C (VEGF-C) in esophageal squamous cell carcinoma (ESCC) by knocking down VEGF-C expression in the ESCC cell line EC9706. METHODS: Immunohistochemistry and in situ hybridization techniques were used to detect the expression of VEGF-C expression in ESCC tissues. We also investigated the relationship between VEGF-C expression and lymph node metastasis. We designed a siRNA expression plasmid for VEGF-C and transfected it into EC9706 cells. Stable clones were selected, and VEGF-C expression was analyzed by RT-PCR and western blotting. Cells were inoculated into nude mice. The expression of VEGF-C in the resulting tumors was analyzed by immunohistochemistry and in situ hybridization. RESULTS: VEGF-C is highly expressed in ESCC and correlated with lymph node metastasis, as high levels were observed in patients presenting with lymph node metastases relative to those who did not (P < 0.01). Transfection with VEGF-C-siRNA decreased the expression of VEGF-C mRNA and protein. ESCC cells stably transfected with VEGF-C-siRNA expressed very low levels of VEGF-C (P < 0.01 compared with control). This knockdown effect persisted when the cells were inoculated into nude mice and allowed to form tumors. CONCLUSIONS: The siRNA-targeted knockdown of VEGF-C led to a significant reduction in VEGF-C expression. This siRNA technique could be used for gene therapy in ESCC. |
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Authors:
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Hongxin Zhang; Yuhui Yin; Lan Zhang; Xiangyu Zheng; Dongling Gao; Kuisheng Chen; Yunhan Zhang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-6 |
Journal Detail:
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Title: Journal of cancer research and clinical oncology Volume: - ISSN: 1432-1335 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902060 Medline TA: J Cancer Res Clin Oncol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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