Document Detail

The effects of ultrafiltration on e-aminocaproic acid: an in vitro analysis.
MedLine Citation:
PMID:  12395966     Owner:  NLM     Status:  MEDLINE    
Blood conservation strategies have become a standard of practice in cardiac surgery, with the use of antifibrinolytic agents and ultrafiltration two popular techniques. The purpose of this study was to evaluate the effects of continuous ultrafiltration on e-aminocaproic acid (EACA) utilizing functional coagulation analysis. A fibrinolytic assay was developed to detect EACA using the thromboelastograph (TEG) and urokinase (0.138 units 0.360 mL(-1)). Fresh bovine blood (23 +/- 1% hematocrit) was pumped (100 mL min(-1)) through an ultrafiltrator (HPH 400) at 37 degrees C with a transmembrane pressure of 280 mmHg. EACA (0.065 mg mL(-1)) was circulated for 10 minutes before initiating ultrafiltration. Samples (pre- and postultrafiltrator) were obtained at baseline, 5, and 10 min of ultrafiltration and analyzed via the fibrinolytic assay for EACA determination. TEG profiles significantly decreased from concentrations of 0.065 mg to 0.0325 mg of EACA mL(-1) blood (maximum amplitude MA, 75.4 +/- 4.0 versus 63.3 +/- 2.9, p < .05, TEG index 5.4 +/- 0.7 versus 4.0 +/- 0.3, p < .05). Fibrinolysis at 30 min increased as EACA concentrations declined (0.065 mg, 0% versus 0.032 mg, 16.4 +/- 2.8%, p < .05). During ultrafiltration the MA increased significantly from baseline to 10 min postultrafiltrator (68.2 +/- 3.0 versus 75.8 +/- 10.0, p < .05) and from 5 min pre- to 10 min postultrafiltrator (69.7 +/- 4.2 versus 75.8 +/- 10.0, p < .05). The TEG index showed no significant change, and no fibrinolysis was detected at 30 min from any datapoint during ultrafiltration. In conclusion, this study demonstrates that the antifibrinolytic properties of EACA are maintained during ultrafiltration with a 25% reduction in total circulating volume.
Craig M Petterson; Alfred H Stammers; Ryan J Kohtz; Scott A Kmiecik; Jeffrey D Nichols; Nancy J Mills; Jun-Li Liu
Related Documents :
19092666 - Hemodynamic energy delivery of the pulsatile flow in a simulated pediatric extracorpore...
3722076 - Vacuum-assisted perfusion of the dog hindlimb.
15823696 - Cardiac torsion and electromagnetic fields: the cardiac bioinformation hypothesis.
2261016 - Left ventricular assistance with the centrifugal pump: management of the patient with s...
22296576 - Wound outcomes in patients with advanced illness.
18189686 - Aero-acoustic noise generation of a lawnmower deck.
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of extra-corporeal technology     Volume:  34     ISSN:  0022-1058     ISO Abbreviation:  J Extra Corpor Technol     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-10-24     Completed Date:  2002-11-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0267637     Medline TA:  J Extra Corpor Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  197-202     Citation Subset:  T    
Division of Clinical Perfusion Education, School of Allied Health Professions, University of Nebraska Medical Center, Omaha, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
6-Aminocaproic Acid / analysis*,  blood
Thoracic Surgery*
United States
Reg. No./Substance:
60-32-2/6-Aminocaproic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Expanding perfusion services through mobile point-of-care coagulation monitoring.
Next Document:  The effect of controlled aprotinin administration through cardiotomy suction during cardiopulmonary ...