Document Detail


The effects of two disulphides on cholinesterase activity in the spectrophotometric assay.
MedLine Citation:
PMID:  4478065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. 5,5'-Dithiobis-(2-nitrobenzoate) did not influence serum cholinesterase activity, whereas 2,2'-dithiodipyridine had an inhibitory effect. 2. The lowering of the molar extinction coefficients observed in the presence of physostigmine may be a result of a reaction between thiolate ions with carbamate moieties. 3. The use of 5,5'-dithiobis-(2-nitrobenzoate) is still recommended in investigations, especially where the quantitative aspects are significant.
Authors:
K B Augustinsson; H Eriksson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  139     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1974 Apr 
Date Detail:
Created Date:  1975-10-03     Completed Date:  1975-10-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  123-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylcholinesterase / blood*
Animals
Butyrylcholinesterase / blood
Cholinesterases / blood*
Disulfides / pharmacology*
Dithionitrobenzoic Acid / pharmacology*
Erythrocytes / enzymology
Horses
Kinetics
Nitrobenzoates / pharmacology*
Physostigmine / pharmacology
Pyridines / pharmacology*
Time Factors
Chemical
Reg. No./Substance:
0/Disulfides; 0/Nitrobenzoates; 0/Pyridines; 57-47-6/Physostigmine; 69-78-3/Dithionitrobenzoic Acid; EC 3.1.1.-/Butyrylcholinesterase; EC 3.1.1.7/Acetylcholinesterase; EC 3.1.1.8/Cholinesterases
Comments/Corrections

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