Document Detail


The effects of tritium on embryo development: the embryotoxic effects of [3H]tryptophan.
MedLine Citation:
PMID:  2569016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of tritiated amino acids on the development of the early mouse embryo in vitro are described. [3H]tryptophan was extremely toxic, compared with [3H]glutamic acid, and inhibited growth of the two-cell embryo to the blastocyst stage. The LD50 values for the two amino acids were, respectively, 1 and 296 kBq/ml of medium. The two-cell embryo was more sensitive to the irradiation from incorporated [3H]tryptophan is selectively incorporated into two chromosomal proteins in the two-cell embryo, and that those proteins were stage-specific markers of development.
Authors:
H M Killen; J Carroll
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  International journal of radiation biology     Volume:  56     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-09-05     Completed Date:  1989-09-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  139-49     Citation Subset:  IM; S    
Affiliation:
Department of Biochemistry, Trinity College, Dublin, Republic of Ireland.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Blastocyst / radiation effects*
Cell Compartmentation
Chromosomes / metabolism
Cleavage Stage, Ovum / radiation effects*
Embryo Implantation
Female
Glutamates / metabolism
Glutamic Acid
Leucine / metabolism
Mice / embryology*
Molecular Weight
Nuclear Proteins / biosynthesis
Protein Biosynthesis
RNA, Messenger / metabolism
Tritium / toxicity*
Tryptophan / metabolism*
Chemical
Reg. No./Substance:
0/Glutamates; 0/Nuclear Proteins; 0/RNA, Messenger; 10028-17-8/Tritium; 56-86-0/Glutamic Acid; 61-90-5/Leucine; 73-22-3/Tryptophan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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