Document Detail


The effects of triclosan on uropathogen susceptibility to clinically relevant antibiotics.
MedLine Citation:
PMID:  18937596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Triclosan is a broad-spectrum antimicrobial agent currently used in numerous products including surgical scrubs and ureteral stents. Unfortunately, studies have shown triclosan resistance among several bacterial species. Our objective was to characterize resistance patterns of common uropathogens to triclosan and determine whether triclosan exposure would alter their susceptibility to common antibiotics. We hypothesized that triclosan exposure induces a metabolic stress rendering some bacterial strains more susceptible to other antibiotics. METHODS: Using largely clinical isolates comprising seven uropathogenic species, we conducted 24 hour growth experiments to determine triclosan minimal inhibitory concentrations (MIC) for each strain. Based upon these MICs, triclosan was added to agar plates at escalating sublethal concentrations and antibiotic disk diffusion assays were conducted using a range of clinically-relevant antibiotics. RESULTS: Varying susceptibility patterns were observed across all antibiotics studied. Several antibiotics demonstrated increased efficacy in conjunction with triclosan. The combined effect of triclosan with amoxicillin and gentamicin was superior when considering significant increases in susceptibility, with 6 (86%) and 5 (71%) of the 7 bacterial strains displaying enhanced sensitivity, respectively. The antimicrobial effects of nitrofurantoin and the fluoroquinolones were significantly enhanced for 4 (57%), 4 and 3 (42%) of the 7 pathogens, respectively. The two fluoroquinolones were the only antibiotics where susceptibility was negatively impacted (in one strain each) in combination with triclosan. CONCLUSIONS: The synergistic effects of triclosan and several antibiotics are consistent with a triclosan-dependent metabolic strain and/or membrane disruptive effect, and offers important insight into the combined use of antimicrobial compounds in clinical practice.
Authors:
Geoffrey R Wignall; Lee W Goneau; Ben H Chew; John D Denstedt; Peter A Cadieux
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of endourology / Endourological Society     Volume:  22     ISSN:  1557-900X     ISO Abbreviation:  J. Endourol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-21     Completed Date:  2008-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807503     Medline TA:  J Endourol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2349-56     Citation Subset:  IM    
Affiliation:
Department of Surgery (Division of Urology), Schulich School of Medicine and Dentistry, University of Western Ontario, Larson Health Research Institute, London, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology*
Anti-Infective Agents, Local / pharmacology*
Bacteria / drug effects*
Biological Assay
Diffusion
Drug Resistance, Microbial*
Microbial Sensitivity Tests
Triclosan / pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Anti-Infective Agents, Local; 3380-34-5/Triclosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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