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The effects of topically applied polyNIPAM-based nanogels and their monomers on skin cyclooxygenase expression, ex vivo.
MedLine Citation:
PMID:  23194376     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Stimulus-responsive nanogels have potential as carriers for drugs targeting the skin. It is important to estimate the biocompatibility of such materials with the skin since they are directly in contact upon application and may induce irritation or inflammation. In the current work, blank (drug-free) polyN-isopropylacrylamide (polyNIPAM), poly(NIPAM copolymerised butyl acrylate) [poly(NIPAM-co-BA)], and poly(NIPAM copolymerized acrylic acid)(5%) [poly(NIPAM-co-AAc)(5%)] nanogels were dosed onto freshly excised porcine ear skin membranes and the effects on the expression on cyclooxygenase-2 (COX-2) determined ex vivo by Western blotting. Modulated COX-2 expression was indicative that the material had penetrated the skin and keratinocytes of the viable epidermis. The poly(NIPAM-co-BA) nanogel was found to exert a proinflammatory response when applied topically, as reflected by 67% higher COX-2 expression relative to the control treatment (p 0.0035). The data obtained for the poly(NIPAM-co-AAc)(5%) nanogel, on the other hand, indicated no significant modulation in the expression of COX-2 (p 0.1578), suggest the particles are compatible with skin. This was even the case in the presence of co-administered aqueous citric acis solution. Overall the data support the use of the multi-responsive poly(NIPAM-co-AAc)(5%) nanogel for triggered or controlled topical drug delivery applications.
Authors:
Charles Heard; Nor Hayati Abu Samah
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-30
Journal Detail:
Title:  Nanotoxicology     Volume:  -     ISSN:  1743-5404     ISO Abbreviation:  Nanotoxicology     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233132     Medline TA:  Nanotoxicology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Cardiff University, School of Pharmacy , King Edward VII Avenue, Redwood, Cardiff, Cardiff, CF10 3NB , +442920875819 , +442910874149 , United Kingdom.
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