Document Detail


The effects of thymidine and its analogues on the differentiation of antibody-forming cells.
MedLine Citation:
PMID:  126887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thymidine (Tdr) and its analogues 5-fluorodeoxyuridine (FUdr) and 5-bromodeoxyuridine (BUdr) inhibited the development of antibody-producing cells (PFC) in cultures of explants of rabbit spleen stimulated with sheep red cells in vitro. They were effective in this respect, even at concentrations too low to inhibit cell division. The results indicated that Tdr had produced its effects by a selective action on DNA and RNA synthesis. In view of what is known about the mechanisms of action of Tdr and its analogues to interfere with DNA-dependent events in cells, these results supported the idea that DNA synthesis, unrelated to the needs for cell division, was required for the development of antibody-producing capacity by PFC in these cultures.
Authors:
G Harris; E M Walford; I Olsen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  3     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1975 Aug 
Date Detail:
Created Date:  1976-01-10     Completed Date:  1976-01-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  99-105     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Formation / drug effects
Antibody-Producing Cells / drug effects*
Bromodeoxyuridine / pharmacology*
Cell Differentiation / drug effects*
Cells, Cultured
DNA / biosynthesis
Dactinomycin / pharmacology
Female
Floxuridine / pharmacology*
Leucine / metabolism
Male
Puromycin / pharmacology
RNA / biosynthesis
Rabbits
Sheep
Spleen / cytology
Thymidine / pharmacology*
Tritium
Uridine / metabolism
Chemical
Reg. No./Substance:
10028-17-8/Tritium; 50-76-0/Dactinomycin; 50-89-5/Thymidine; 50-91-9/Floxuridine; 53-79-2/Puromycin; 58-96-8/Uridine; 59-14-3/Bromodeoxyuridine; 61-90-5/Leucine; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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