Document Detail

The effects of thymidine and its analogues on the differentiation of antibody-forming cells.
MedLine Citation:
PMID:  126887     Owner:  NLM     Status:  MEDLINE    
Thymidine (Tdr) and its analogues 5-fluorodeoxyuridine (FUdr) and 5-bromodeoxyuridine (BUdr) inhibited the development of antibody-producing cells (PFC) in cultures of explants of rabbit spleen stimulated with sheep red cells in vitro. They were effective in this respect, even at concentrations too low to inhibit cell division. The results indicated that Tdr had produced its effects by a selective action on DNA and RNA synthesis. In view of what is known about the mechanisms of action of Tdr and its analogues to interfere with DNA-dependent events in cells, these results supported the idea that DNA synthesis, unrelated to the needs for cell division, was required for the development of antibody-producing capacity by PFC in these cultures.
G Harris; E M Walford; I Olsen
Related Documents :
23201097 - Experimental and clinical factors influencing long-term stable in vitro expansion of mu...
3936827 - Uv-induced unscheduled dna synthesis in cultured human epidermal and dermal cells.
6330977 - Reversible restriction of vesicular stomatitis virus in permissive cells treated with i...
24434767 - Multistructural biomimetic substrates for controlled cellular differentiation.
17064687 - Inhibitory effects of homeodomain-interacting protein kinase 2 on the aorta-gonad-meson...
23201097 - Experimental and clinical factors influencing long-term stable in vitro expansion of mu...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  3     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1975 Aug 
Date Detail:
Created Date:  1976-01-10     Completed Date:  1976-01-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  99-105     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibody Formation / drug effects
Antibody-Producing Cells / drug effects*
Bromodeoxyuridine / pharmacology*
Cell Differentiation / drug effects*
Cells, Cultured
DNA / biosynthesis
Dactinomycin / pharmacology
Floxuridine / pharmacology*
Leucine / metabolism
Puromycin / pharmacology
RNA / biosynthesis
Spleen / cytology
Thymidine / pharmacology*
Uridine / metabolism
Reg. No./Substance:
10028-17-8/Tritium; 50-76-0/Dactinomycin; 50-89-5/Thymidine; 50-91-9/Floxuridine; 53-79-2/Puromycin; 58-96-8/Uridine; 59-14-3/Bromodeoxyuridine; 61-90-5/Leucine; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Adoption and foster care of handicapped children in the United States.
Next Document:  Isolated tricuspid insufficiency of traumatic type. Report of a case and revision of the literature ...