Document Detail

The effects of tetradecyl-4-ethyl-pyridinium chloride on the maximum specific growth rate biomass and hydrolytic enzyme production of Bacteroides gingivalis in continuous culture.
MedLine Citation:
PMID:  2079447     Owner:  NLM     Status:  MEDLINE    
Bacteroides gingivalis was grown in continuous culture in the presence of tetradecyl-4-ethyl-pyridinium chloride (TDEPC). The maximum specific growth rate and biomass levels decreased with increasing concentrations of antimicrobial and complete inhibition of growth occurred when the TDEPC concentration reached 40 mg/l. Hydrolytic enzymes were detected in cells, vesicles and supernatant fractions of whole culture. Levels of alkaline phosphatase initially increased with increasing concentrations of TDEPC, but at higher concentrations (15-20 mg/l) of antimicrobial decreased significantly to less than 20% of the control value. The levels of N-acetyl-beta-glucosaminidase remained approximately constant at lower concentrations of TDEPC (0 10 mg/l) but then decreased significantly at higher concentrations. In contrast, levels of trypsin-like protease were reduced significantly at even low concentrations of TDEPC (5 10 mg/l) and decreased further as the TDEPC concentration increased. Therefore, TDEPC exerts significant physiological effects on B. gingivalis at concentrations below those considered to be lethal to the cell.
J Greenman; T Minhas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of antimicrobial chemotherapy     Volume:  26     ISSN:  0305-7453     ISO Abbreviation:  J. Antimicrob. Chemother.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-05-01     Completed Date:  1991-05-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7513617     Medline TA:  J Antimicrob Chemother     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  649-57     Citation Subset:  IM    
Department of Science, Bristol Polytechnic, Frenchay, UK.
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MeSH Terms
Acetylglucosaminidase / metabolism
Alkaline Phosphatase / metabolism
Anti-Bacterial Agents / pharmacology*
Bacteroides / drug effects,  enzymology*,  growth & development
Culture Media
Pyridinium Compounds / pharmacology*
Regression Analysis
Trypsin / metabolism
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Culture Media; 0/Pyridinium Compounds; 35014-84-7/tetradecyl-4-ethylpyridinium; EC Phosphatase; EC; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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