Document Detail


The effects of subconjunctival verapamil on filtering blebs in rabbits.
MedLine Citation:
PMID:  8860605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVE: Verapamil is a calcium antagonist that has been shown to modulate wound healing through multiple mechanisms. The wound modulating effects of verapamil were studied in a rabbit model of filtering surgery. MATERIALS AND METHODS: Twenty New Zealand albino rabbits underwent a fistulizing procedure, with either verapamil or saline injected subconjunctivally daily for 6 days following surgery. The animals were sacrificed at 20 days after the fistulizing procedure. The effectiveness of verapamil in modulating wound healing was evaluated by bleb patency testing, histology, measurement of scar thickness, and counts of bromodeoxyuridine (BrdU)-labeled cells at the sclerostomy site. RESULTS: The group treated with verapamil had a 44 percent patency rate compared with 10 percent in the control group (P < .05), a mean scar thickness of 195.96 +/- 68 mu m compared with 313.55 +/- 110.4 mu m in the control group (P < .02), and a mean BrdU-labeled cell count of 15.5 +/- 14.7 compared with 26.3 +/- 17.0 in the control group (P < .002). CONCLUSION: Verapamil was effective in reducing scar formation at the sclerostomy site. At the doses administered, verapamil appeared to modulate wound healing in filtering blebs in the rabbit model. Further studies are needed to determine the dose-response and the efficacy of the drug in nonhuman primates.
Authors:
B Gupta; A Agarwala; J J Moy; D P Edward
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Ophthalmic surgery and lasers     Volume:  27     ISSN:  1082-3069     ISO Abbreviation:  Ophthalmic Surg Lasers     Publication Date:  1996 May 
Date Detail:
Created Date:  1997-05-19     Completed Date:  1997-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9517132     Medline TA:  Ophthalmic Surg Lasers     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  378-83     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Eye and Ear Infirmary, University of Illinois at Chicago, Ill, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium Channel Blockers / administration & dosage,  pharmacology*
Cell Division
Conjunctiva
DNA Replication / drug effects
Disease Models, Animal
Dose-Response Relationship, Drug
Follow-Up Studies
Injections
Rabbits
Sclera / drug effects*,  pathology
Sclerostomy*
Treatment Outcome
Verapamil / administration & dosage,  pharmacology*
Wound Healing / drug effects
Chemical
Reg. No./Substance:
0/Calcium Channel Blockers; 52-53-9/Verapamil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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