|The effects of selective COX-2 inhibitor/celecoxib and omega-3 fatty acid on matrix metalloproteinases, TIMP-1, and laminin-5gamma2-chain immunolocalization in experimental periodontitis.|
|PMID: 18834249 Owner: NLM Status: MEDLINE|
|BACKGROUND: Matrix metalloproteinases (MMPs) play important roles in tissue-destruction mechanisms-associated periodontitis. MMP-8 and -13 are the predominant collagenases that are important in the extracellular matrix degradation in periodontal tissues. MMP-14 is a membrane-type MMP, whereas laminin-5 indicates basal membrane modification and epithelial induction. The purpose of the present study was to evaluate the effects of celecoxib and omega-3 fatty acid administration on the gingival tissue expression of MMP-8, -13, and -14, tissue inhibitor of MMP (TIMP)-1, and laminin (Ln)-5gamma2-chain in rat experimental periodontitis induced by Escherichia coli endotoxin (lipopolysaccharide [LPS]). METHODS: Experimental periodontitis was induced in rats by repeated LPS injection. Fifty-one adult male Sprague-Dawley rats were divided into six study groups: saline control, LPS, LPS + celecoxib, LPS + therapeutic omega-3 (TO3), prophylactic omega-3 + LPS + omega-3 (P+TO3), and LPS + celecoxib + omega-3 fatty acid. Celecoxib and omega-3 fatty acid were given as a single agent or as combination therapy for 14 days. On day 15, all rats were sacrificed, and gingival tissues were analyzed immunohistochemically for the expression of MMP-8, -13, and -14, TIMP-1, and Ln-5gamma2-chain. Alveolar bone loss was evaluated morphometrically under a stereomicroscope. Data were tested statistically by Kruskal-Wallis and Mann-Whitney tests and Spearman correlation analysis. RESULTS: Alveolar bone loss was significantly higher in all study groups compared to the saline control group (all P <0.01). MMP-8 expression was significantly higher in the LPS group than in the saline group (P = 0.001). Very low expression of MMP-8 was found in the celecoxib, P+TO3, and combination groups. TO3 increased TIMP-1 expression significantly compared to the LPS group (P <0.05). Individual celecoxib and P+TO3 administration increased MMP-14 significantly compared to saline control and LPS groups (P <0.05). No significant differences were found among the study groups with regard to Ln-5gamma2-chain and MMP-13 expressions (P >0.05). CONCLUSIONS: Selective cyclooxygenase-2 inhibitor, prophylactic omega-3 fatty acid, and a combination of these two agents can inhibit gingival tissue MMP-8 expression. Moreover, the individual administration of therapeutic omega-3 may increase gingival TIMP-1 expression in contrast to no effect on MMP-8, -13, and -14 expressions in experimental periodontitis. These experimental findings in a rat model of LPS-induced periodontitis need to be verified by clinical human studies.|
|Saynur Vardar-Sengul; Eralp Buduneli; Oya Turkoglu; Nurcan Buduneli; Gül Atilla; Jaana Wahlgren; Timo Sorsa; Haluk Baylas|
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|Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't|
|Title: Journal of periodontology Volume: 79 ISSN: 0022-3492 ISO Abbreviation: J. Periodontol. Publication Date: 2008 Oct|
|Created Date: 2008-10-06 Completed Date: 2009-01-13 Revised Date: -|
Medline Journal Info:
|Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: United States|
|Languages: eng Pagination: 1934-41 Citation Subset: D; IM|
|Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey. firstname.lastname@example.org|
|APA/MLA Format Download EndNote Download BibTex|
Alveolar Bone Loss
Cyclooxygenase 2 Inhibitors / therapeutic use*
Disease Models, Animal
Fatty Acids, Omega-3 / therapeutic use*
Gingiva / drug effects, enzymology
Gingivitis / drug therapy, enzymology, pathology
Laminin / analysis, drug effects*
Matrix Metalloproteinase 13 / analysis, drug effects
Matrix Metalloproteinase 14 / analysis, drug effects
Matrix Metalloproteinase 8 / analysis, drug effects
Matrix Metalloproteinases / analysis, drug effects*
Periodontitis / drug therapy*, enzymology, pathology
Pyrazoles / therapeutic use*
Sulfonamides / therapeutic use*
Tissue Inhibitor of Metalloproteinase-1 / analysis, drug effects*
|0/Cyclooxygenase 2 Inhibitors; 0/Drug Combinations; 0/Fatty Acids, Omega-3; 0/Laminin; 0/Lipopolysaccharides; 0/Pyrazoles; 0/Sulfonamides; 0/Tissue Inhibitor of Metalloproteinase-1; 169590-42-5/celecoxib; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.-/Mmp13 protein, rat; EC 18.104.22.168/Matrix Metalloproteinase 8; EC 22.214.171.124/Matrix Metalloproteinase 14|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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